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Bridging technique ...
Bridging technique failure through low-tech improvisation: A case study of food microbiology
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- Naznin, Most Tahera (författare)
- Swedish University of Agricultural Sciences
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- Widstam, Sofia (författare)
- Swedish University of Agricultural Sciences
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- Akter, Rumana (författare)
- Swedish University of Agricultural Sciences
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- Mogren, Lars (författare)
- Swedish University of Agricultural Sciences
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- Berhane, Hanna Y (författare)
- Addis Continental Institute of Public Health
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- Berhane, Yemane (författare)
- Addis Continental Institute of Public Health
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- Ekström, Eva-Charlotte (författare)
- Uppsala University
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- Jirström, Magnus (författare)
- Lund University,Lunds universitet,Institutionen för kulturgeografi och ekonomisk geografi,Samhällsvetenskapliga institutioner och centrumbildningar,Samhällsvetenskapliga fakulteten,Department of Human Geography,Departments of Administrative, Economic and Social Sciences,Faculty of Social Sciences
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- Alsanius, Beatrix W. (författare)
- Swedish University of Agricultural Sciences
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(creator_code:org_t)
- 2020
- 2020
- Engelska 5 s.
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Ingår i: African Journal of Microbiology Research. - 1996-0808. ; 14:7, s. 361-365
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Abstract
Ämnesord
Stäng
- Modern technology for food safety studies includes standardized protocols and equipment. However, appropriate technology needs to step in to bridge technology dys- or malfunctioning. We examined different low-tech methods for extraction of bacteria from fresh vegetables. Standard equipment including stomacher and filter bags were compared to extraction using bread stick and alternative filter material (nylon stocking, mosquito net). Comparison of microspheres’ (ø: 53-63 µm; ø: 63-75 µm) passage through filter bags, nylon stockings with different densities (15 DEN, 20 DEN, 25 DEN, 40 DEN) and mosquito net showed no significant difference between filter bag and nylon stocking. A significantly higher number of both size microspheres (ø: 53-63 and ø: 63-75 µm) passed through the mosquito net than filter bag and nylon stocking. Manual extraction of romaine lettuce leaf was performed by three technicians. Viable counts of leaf associated bacteria were influenced by the technician and choice of filter material. Viable bacterial counts obtained from breadstick with filter bag manual extraction did not show any significant difference from standard method. We conclude that standard procedures can be replaced by low-tech approaches in the event of malfunctioning equipment. However, method validation is imperative.
Ämnesord
- SAMHÄLLSVETENSKAP -- Annan samhällsvetenskap -- Tvärvetenskapliga studier inom samhällsvetenskap (hsv//swe)
- SOCIAL SCIENCES -- Other Social Sciences -- Social Sciences Interdisciplinary (hsv//eng)
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Livsmedelsvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Food Science (hsv//eng)
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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