Solid-liquid interface method (SLIM) : a new crystallization method for proteins

• Despite impressive advances in theories, methods and technologies, crystallization still remains a serious bottleneck in structural determination of macromolecules. Here we present a novel solid-liquid interface method (SLIM) for protein crystallization, based on the pre-adding and drying of a crystallization reagent, and thereafter the dispensing of a protein solution to the dried media to initiate crystallization from the solid-liquid interface. Not only quick and easy to perform, the method also allows for a less concentrated protein solution for setting up crystallization trials.

Nyckelord

Crystallization

Proteins

Publikations- och innehållstyp

art (ämneskategori)

ref (ämneskategori)