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Micro- and nanostru...
Micro- and nanostructure specific X-ray tomography reveals less matrix formation and altered collagen organization following reduced loading during Achilles tendon healing
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- Silva Barreto, Isabella (författare)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,LTH profilområde: Teknik för hälsa,LTH profilområden,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,LTH Profile Area: Engineering Health,LTH Profile areas,Faculty of Engineering, LTH,Lund Univ, Sweden
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- Pierantoni, Maria (författare)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,LTH profilområde: Teknik för hälsa,LTH profilområden,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,LTH Profile Area: Engineering Health,LTH Profile areas,Faculty of Engineering, LTH,Lund Univ, Sweden
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- Nielsen, Leonard, 1992 (författare)
- Chalmers University of Technology,Chalmers tekniska högskola,Chalmers Univ Technol, Sweden
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- Hammerman, Malin (författare)
- Linköping University,Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten,Lund Univ, Sweden
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- Diaz, Ana (författare)
- Paul Scherrer Institute,Paul Scherrer Institut,Paul Scherrer Inst, Switzerland
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- Novak, Vladimir (författare)
- Paul Scherrer Institute,Paul Scherrer Institut,Paul Scherrer Inst, Switzerland
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- Eliasson, Pernilla T. (författare)
- Linköping University,Sahlgrenska University Hospital,Sahlgrenska universitetssjukhuset,Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten,Sahlgrens Univ Hosp, Sweden
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- Liebi, Marianne (författare)
- Chalmers University of Technology,Swiss Federal Institute of Technology,Paul Scherrer Institute,Chalmers tekniska högskola,Ecole Polytechnique Federale de Lausanne (EPFL),Swiss Federal Institute of Technology in Lausanne (EPFL),Paul Scherrer Institut,Chalmers Univ Technol, Sweden; Paul Scherrer Inst, Switzerland; Ecole Polytech Fed Lausanne EPFL, Switzerland
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- Isaksson, Hanna (författare)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,LTH profilområde: Teknik för hälsa,LTH profilområden,LU profilområde: Proaktivt åldrande,Lunds universitets profilområden,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,LTH Profile Area: Engineering Health,LTH Profile areas,Faculty of Engineering, LTH,LU Profile Area: Proactive Ageing,Lund University Profile areas,Lund Univ, Sweden
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(creator_code:org_t)
- ELSEVIER SCI LTD, 2024
- 2024
- Engelska 13 s.
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Ingår i: Acta Biomaterialia. - : ELSEVIER SCI LTD. - 1742-7061 .- 1878-7568. ; 174, s. 245-257
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://research.cha... (primary) (free)
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Recovery of the collagen structure following Achilles tendon rupture is poor, resulting in a high risk for re-ruptures. The loading environment during healing affects the mechanical properties of the tendon, but the relation between loading regime and healing outcome remains unclear. This is partially due to our limited understanding regarding the effects of loading on the micro- and nanostructure of the healing tissue. We addressed this through a combination of synchrotron phase-contrast X-ray microtomography and small-angle X-ray scattering tensor tomography (SASTT) to visualize the 3D organization of microscale fibers and nanoscale fibrils, respectively. The effect of in vivo loading on these structures was characterized in early healing of rat Achilles tendons by comparing full activity with immobilization. Unloading resulted in structural changes that can explain the reported impaired mechanical performance. In particular, unloading led to slower tissue regeneration and maturation, with less and more disorganized collagen, as well as an increased presence of adipose tissue. This study provides the first application of SASTT on soft musculoskeletal tissues and clearly demonstrates its potential to investigate a variety of other collagenous tissues. Statement of significance: Currently our understanding of the mechanobiological effects on the recovery of the structural hierarchical organization of injured Achilles tendons is limited. We provide insight into how loading affects the healing process by using a cutting-edge approach to for the first time characterize the 3D micro- and nanostructure of the regenerating collagen. We uncovered that, during early healing, unloading results in a delayed and more disorganized regeneration of both fibers (microscale) and fibrils (nanoscale), as well as increased presence of adipose tissue. The results set the ground for the development of further specialized protocols for tendon recovery.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biomaterial (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bio Materials (hsv//eng)
Nyckelord
- 3D organization
- Fibers
- Fibrils
- Hierarchical tissue structure
- Phase-contrast microtomography
- Small-angle X-ray scattering tensor tomography
- Fibrils
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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