Evidence that Hy-RBCs express weak Jo(a) antigen

• BACKGROUND: RBCs of the Hy- phenotype have, in the past, been typed as Gy(a+(w)), Hy-, Jo(a-), and RBCs with the Jo(a-) phenotype type Gy(a+), Hy-(w), and Jo(a-). Anti-Hy and anti-Jo(a) are difficult to identify mainly because appropriate reagent R6Cs are poorly characterized. Historically, anti-Jo(a) has not reacted with RBCs with either phenotype. This report describes a case of an anti-Jo(a) that shows Hy- RBCs express some Jo(a) antigen, albeit weakly. CASE REPORT: Anti-Jo(a) was identified in a serum sample of a 71-year-old woman. The antibody reacted 1+ to 2+ by the IAT with all untreated and ficin-treated panel RBCs and did not react with Gy(a-) RBCs and Jo(a-) RBCs. Unexpectedly, the serum sample reacted weakly with six of eight RBC samples with the Hy- phenotype. The anti_Jo(a) was adsorbed onto and eluted from Hy- RBCs, indicating the presence of weak Jo(a) antigen. The patient's RBCs typed Gy(a+), Hy+, Jo(a-). DNA studies using PCR-RFLP analysis showed the patient to be homozygous for the JO allele, which is consistent with the serologically determined Jo(a-) status. CONCLUSION: The DNA and serologic evidence of this case show that Hy- RBCs may express low levels of Jo(a) antigen, which contradicts previously published data concerning the Jo(a) type of Hy- RBCs.

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MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Hematologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Hematology (hsv//eng)

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