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Cyclosporin A and i...
Cyclosporin A and its nonimmunosuppressive analogue N-Me-Val-4-cyclosporin A mitigate glucose/oxygen deprivation-induced damage to rat cultured hippocampal neurons
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- Khaspekov, Leonid (författare)
- Lund University,Russian Academy of Medical Science
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- Friberg, Hans (författare)
- Lund University,Lunds universitet,Laboratory for Experimental Brain Research,Forskargrupper vid Lunds universitet,Lund University Research Groups,Skåne University Hospital
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- Halestrap, Andrew (författare)
- University of Bristol
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- Viktorov, Ilja (författare)
- Russian Academy of Medical Science
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- Wieloch, Tadeusz (författare)
- Lund University,Lunds universitet,Laboratory for Experimental Brain Research,Forskargrupper vid Lunds universitet,Lund University Research Groups
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(creator_code:org_t)
- 2001-12-24
- 1999
- Engelska 5 s.
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Ingår i: European Journal of Neuroscience. - : Wiley. - 0953-816X. ; 11:9, s. 3194-3198
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- When mouse hippocampal neuronal cultures, 2-3 weeks in vitro, were transiently exposed to combined glucose and oxygen deprivation (100% argon, 5% CO2, in glucose-free medium) for 90 min, extensive neuronal degeneration had occurred after 24 h of reoxygenation. When these cultures were preincubated with cyclosporin A, a calcineurin inhibitor and a blocker of the mitochondrial permeability transition, neuronal death diminished by 30-50%. Similarly, the cyclosporin A analogue, N-Me-Val-4-cyclosporin A, a potent blocker of the mitochondrial permeability transition with no significant calcineurin blocking activity, decreased cell death by 70-80%. Both cyclosporin A and N-Me-Val-4-cyclosporin A markedly attenuated calcium-induced swelling of isolated mouse brain mitochondria by blocking the mitochondrial permeability transition. The potassium thiocyanate-stabilized binding of cyclophilin D to mouse brain mitochondrial membranes was completely prevented by cyclosporin A and N-Me-Val-4-cyclosporin A. Our results strongly suggest that the mitochondrial permeability transition is involved in oxygen/glucose deprivation-induced cell death in vitro. Cyclophilin D and other components of the mitochondrial permeability transition may be important targets for neuroprotective and anti-ischaemic drugs.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Nyckelord
- Apoptosis
- Cell cultures
- Mitochondrial permeability transition
- Neuronal death
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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