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Real-time Fluoresce...
Real-time Fluorescence Imaging of Capillary Electrophoresis
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Nilsson, S (författare)
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- Johansson, Jonas (författare)
- Lund University,Lunds universitet,Atomfysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Atomic Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH
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Mecklenburg, M (författare)
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visa fler...
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Birnbaum, S (författare)
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- Svanberg, Sune (författare)
- Lund University,Lunds universitet,Atomfysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Atomic Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH
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Wahlund, K. G (författare)
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Mosbach, K (författare)
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Miyabayashi, A (författare)
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- Larsson, P. O (författare)
- Lund University,Lunds universitet,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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visa färre...
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(creator_code:org_t)
- 1995
- 1995
- Engelska 7 s.
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Ingår i: Journal of Capillary Electrophoresis. - 1079-5383. ; 2:1, s. 46-52
- Relaterad länk:
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https://lup.lub.lu.s...
Abstract
Ämnesord
Stäng
- An arrangement for real-time fluorescence imaging of capillary electrophoretic separations is described. A decoated capillary was excited along a substantial part of its length by a dye laser pumped with an XeCl excimer laser tuned to match the fluorophors in the sample. Light emitted from the migrating, fluorescent molecules was detected by an image-intensified, thermoelectrically cooled charge-coupled device camera. The camera signals were processed by a computer and displayed as moving peaks on a screen in real-time, thus allowing the progress of the separation to be monitored continuously. Basic characteristics of the imaging arrangement such as sensitivity (signal-to-noise ratio) and resolution were checked with fluorescein samples. The imaging principle was also illustrated with a separation of a mixture of DNA fragments on a commercially available capillary designated for DNA separations. The benefits of real-time imaging for separation processes, in particular for capillary electrophoresis, are discussed.
Ämnesord
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
Nyckelord
- Capillary electrophoresis
- Real-time imaging
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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