Regulation of bone sialoprotein mRNA by steroid hormones.

• In this report we demonstrate an increase in the steady-state level of bone sialoprotein (BSP) mRNA in rat calvaria and a rat osteosarcoma cell line (ROS 17/2.8) after treatment with the synthetic glucocorticoid, dexamethasone. In contrast, 1.25-dihydroxyvitamin D3 reduced the amount of BSP mRNA in calvaria and inhibited the dexamethasone induction in ROS 17/2.8 cells. The increase in BSP mRNA is most likely due to an increase in the transcriptional rate. The stability of mRNA was unchanged after dexamethasone treatment with a half-life of approximately 5 h. Nuclear transcription experiments with nuclei isolated from ROS 17/2.8 cells showed an increased BSP mRNA synthesis in cells treated with dexamethasone.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Oto-rhino-laryngologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Otorhinolaryngology (hsv//eng)

Nyckelord

Messenger: genetics

Messenger: drug effects

RNA

Genes: drug effects

Gene Expression Regulation: drug effects

Dexamethasone: pharmacology

Calcitriol: pharmacology

Bone and Bones: drug effects

Bone and Bones: metabolism

Sialoglycoproteins: genetics

Transcription

Genetic: drug effects

Publikations- och innehållstyp

art (ämneskategori)

ref (ämneskategori)