氨曲南竞争性抑制NDM-1对β-内酰胺类抗生素的水解

• We investigated the effect of Aztreonam on hydrolysis of β-lactam antibiotics by MBL using New Delhi Metallo-β-lactamase-1(NDM-1) as a model. The results showed that Aztreonam significantly inhibited hydrolysis of Nitrocefin and Meropenem by soluble NDM-1, but also inhibited hydrolysis of Penicillin G by CPG beads immobilized NDM-1(NDM-1 beads). Moreover, in spite of extensive washing for multiple times, the activity to hydrolyze Penicillin G by the Aztreoman pre-treated NDM-1 beads was just partially recovered. These data suggest that Aztreonam can covalently and stably bind on NDM-1, thus efficiently inhibiting hydrolysis of other kinds of β-lactam antibiotics by NDM-1 in a competitive way.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

Aztreonam

NDM-1

metal-β-lactamase

Superbug

Antimicrobial resistance

Publikations- och innehållstyp

art (ämneskategori)

ref (ämneskategori)