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Steady-state cyclic...
Steady-state cyclic electron transfer through solubilized Rhodobacter sphaeroides reaction centres
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- van Rotterdam, Bart (author)
- Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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Westerhoff, Hans V (author)
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Visschers, Ronald W (author)
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Jones, Michael R (author)
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Hellingwerf, Klaas J (author)
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Crielaard, Wim (author)
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(creator_code:org_t)
- 2000
- 2000
- English.
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In: Biophysical Chemistry. - 1873-4200. ; 88:1-3, s. 137-152
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http://dx.doi.org/10...
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Abstract
Subject headings
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- The mechanism, thermodynamics and kinetics of light-induced cyclic electron transfer have been studied in a model energy-transducing system consisting of solubilized Rhodobacter sphaeroides reaction center/light harvesting-1 complexes (so-called core complexes), horse heart cytochrome c and a ubiquinone-0/ubiquinol-0 pool. An analysis of the steady-state kinetics of cytochrome c reduction by ubiquinol-0, after a light-induced steady-state electron flow had been attained, showed that the rate of this reaction is primarily controlled by the one-electron oxidation of the ubiquinol-anion. Re-reduction of the light-oxidized reaction center primary donor by cytochrome c was measured at different reduction levels of the ubiquinone-0/ubiquinol-0 pool. These experiments involved single turnover flash excitation on top of background illumination that elicited steady-state cyclic electron transfer. At low reduction levels of the ubiquinone-0/ubiquinol-0 pool, the total cytochrome c concentration had a major control over the rate of reduction of the primary donor. This control was lost at higher reduction levels of the ubiquinone/ubiquinol-pool, and possible reasons for this behaviour are discussed.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- Rhodobacter sphaeroides
- Reaction center
- Electron transfer
- Ubiquinone
- Cytochrome c
- Control theory
Publication and Content Type
- art (subject category)
- ref (subject category)
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