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Targeting hepatocyt...
Targeting hepatocytes from liver tissue by laser capture microdissection and proteomics expression profiling.
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- Marko-Varga, György (author)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Kemiska institutionen,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Department of Clinical Sciences, Lund,Faculty of Medicine,Department of Chemistry,Faculty of Engineering, LTH
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- Berglund, Magnus (author)
- Lund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Malmström, Johan (author)
- Lund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine
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Lindberg, H. (author)
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Fehniger, T E (author)
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(creator_code:org_t)
- Wiley, 2003
- 2003
- English.
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In: Electrophoresis. - : Wiley. - 0173-0835 .- 1522-2683. ; 24:21, s. 3800-3805
- Related links:
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http://www.ncbi.nlm....
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Subject headings
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- A tissue proteomics process is presented where hepatocyte cell isolation in combination with two-dimensional (2-D) gel electrophoresis and mass spectrometric identification were used to annotate the liver proteome. Laser microdissection of 8 m liver tissue sections was performed and protein expression profiling was compared using a variety of quantities of input cells, and gel separation conditions. The 30 m diameter laser generated the highest protein yields from the polymer coated caps following microsolubilization. We found that 6000 laser pulses (approximately 7200 hepatocytes) were required in order to generate high-resolution gel maps. Within homogeneous tissue samples, this could be accomplished in a total cycle time of 20 min using an automated dissection procedure. Close to 1000 high-quality gel annotations were generated from the corresponding 2-D gel expression profiles which matched closely the corresponding patterns of analytical-scale liver preparations detected by silver staining.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Matrix-assisted laser desorption/ionization-time of flight
- Laser microdissection
- Liver tissue
- Two-dimensional gel electrophoresis
- Miniaturization
Publication and Content Type
- art (subject category)
- ref (subject category)
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