Identification of L-3,4-dihydroxyphenylalanine in the brain of the crayfish Pacifastacus leniusculus (Dana) and in tentacles of the sea anemone Metridium senile (L) by use of gas chromatography-mass spectrometry.

• 1. The amino acid l-3,4-dihydroxyphenylalanine (l-DOPA) was extracted from tentacles of the sea anemone Metridium senile and from brains of the crayfish Pacifastacus leniuscuius.2. The identity of l-DOPA was ascertained by gas chromatography-mass spectrometry as the heptafluorobutyryl iso-butyl ester derivative. Dopa is identified with GC-MS for the first time and the derivatization is a novel modification.3. The results display a capability of invertebrate neurons to accumulate l-DOPA, the precursor of biogenic amines, like the biogenic amines themselves.

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NATURVETENSKAP  -- Biologi -- Zoologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Zoology (hsv//eng)

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