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Oleic acid is a key...
Oleic acid is a key cytotoxic component of HAMLET-like complexes
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- Permyakov, Sergei E (författare)
- P.N. Lebedev Physical Institute of the Russian Academy of Sciences
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- Knyazeva, Ekaterina L (författare)
- P.N. Lebedev Physical Institute of the Russian Academy of Sciences
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- Khasanova, Leysan M (författare)
- P.N. Lebedev Physical Institute of the Russian Academy of Sciences
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- Fadeev, Roman S (författare)
- P.N. Lebedev Physical Institute of the Russian Academy of Sciences
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- Zhadan, Andrei P (författare)
- P.N. Lebedev Physical Institute of the Russian Academy of Sciences
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- Roche-Hakansson, Hazeline (författare)
- The State University of New York
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- Håkansson, Anders P (författare)
- Lund University,Lunds universitet,Experimentell infektionsmedicin, Malmö,Forskargrupper vid Lunds universitet,Experimental Infection Medicine, Malmö,Lund University Research Groups
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- Akatov, Vladimir S (författare)
- P.N. Lebedev Physical Institute of the Russian Academy of Sciences
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- Permyakov, Eugene A (författare)
- P.N. Lebedev Physical Institute of the Russian Academy of Sciences
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PN. Lebedev Physical Institute of the Russian Academy of Sciences The State University of New York (creator_code:org_t)
- 2012
- 2012
- Engelska 8 s.
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Ingår i: Biological Chemistry. - 1437-4315. ; 393:1-2, s. 85-92
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- HAMLET is a complex of α-lactalbumin (α-LA) with oleic acid (OA) that selectively kills tumor cells and Streptococcus pneumoniae. To assess the contribution of the proteinaceous component to cytotoxicity of HAMLET, OA complexes with proteins structurally and functionally distinct from α-LA were prepared. Similar to HAMLET, the OA complexes with bovine β-lactoglobulin (bLG) and pike parvalbumin (pPA) (bLG-OA-45 and pPA-OA-45, respectively) induced S. pneumoniae D39 cell death. The activation mechanisms of S. pneumoniae death for these complexes were analogous to those for HAMLET, and the cytotoxicity of the complexes increased with OA content in the preparations. The half-maximal inhibitory concentration for HEp-2 cells linearly decreased with rise in OA content in the preparations, and OA concentration in the preparations causing HEp-2 cell death was close to the cytotoxicity of OA alone. Hence, the cytotoxic action of these complexes against HEp-2 cells is induced mostly by OA. Thermal stabilization of bLG upon association with OA implies that cytotoxicity of bLG-OA-45 complex cannot be ascribed to molten globule-like conformation of the protein component. Overall, the proteinaceous component of HAMLET-like complexes studied is not a prerequisite for their activity; the cytotoxicity of these complexes is mostly due to the action of OA.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- Animals
- Anti-Bacterial Agents
- Antineoplastic Agents
- Cattle
- Cell Death
- Cell Line, Tumor
- Cell Survival
- Dose-Response Relationship, Drug
- Drug Screening Assays, Antitumor
- Humans
- Lactalbumin
- Lactoglobulins
- Microbial Sensitivity Tests
- Oleic Acid
- Parvalbumins
- Protein Stability
- Streptococcus pneumoniae
- Structure-Activity Relationship
- Temperature
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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