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Functional Role of ...
Functional Role of Glycosaminoglycans in Decellularized Lung Extracellular Matrix
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- Uhl, Franziska (author)
- Lund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine,University of Vermont
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- Zhang, Fuming (author)
- Rensselaer Polytechnic Institute
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- Pouliot, Robert (author)
- University of Vermont
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- Uriarte, Juan Jose (author)
- University of Vermont
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- Rolandsson Enes, Sara (author)
- Lund University,Lunds universitet,Lungbiologi,Forskargrupper vid Lunds universitet,Lung Biology,Lund University Research Groups,University of Vermont
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- Han, Xiaorui (author)
- Rensselaer Polytechnic Institute
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- Ouyang, Yilan (author)
- Rensselaer Polytechnic Institute
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- Xia, Ke (author)
- Rensselaer Polytechnic Institute
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- Westergren-Thorsson, Gunilla (author)
- Lund University,Lunds universitet,Lungbiologi,Forskargrupper vid Lunds universitet,Lund University Bioimaging Center,Medicinska fakulteten,WCMM- Wallenberg center för molekylär medicinsk forskning,Lung Biology,Lund University Research Groups,Faculty of Medicine,WCMM-Wallenberg Centre for Molecular Medicine
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- Malmström, Anders (author)
- Lund University,Lunds universitet,Matrixbiologi,Forskargrupper vid Lunds universitet,WCMM- Wallenberg center för molekylär medicinsk forskning,Medicinska fakulteten,Matrix Biology,Lund University Research Groups,WCMM-Wallenberg Centre for Molecular Medicine,Faculty of Medicine
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- Hallgren, Oskar (author)
- Lund University,Lunds universitet,Lungmedicin, allergologi och palliativ medicin,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Respiratory Medicine, Allergology, and Palliative Medicine,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Linhardt, Robert J (author)
- Rensselaer Polytechnic Institute
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- Weiss, Daniel (author)
- University of Vermont
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(creator_code:org_t)
- Elsevier BV, 2020
- 2020
- English 16 s.
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In: Acta Biomaterialia. - : Elsevier BV. - 1878-7568 .- 1742-7061. ; 102, s. 231-246
- Related links:
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http://dx.doi.org/10...
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https://www.ncbi.nlm...
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Abstract
Subject headings
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- Despite progress in use of decellularized lung scaffolds in ex vivo lung bioengineering schemes, including use of gels and other materials derived from the scaffolds, the detailed composition and functional role of extracellular matrix (ECM) proteoglycans (PGs) and their glycosaminoglycan (GAG) chains remaining in decellularized lungs, is poorly understood. Using a commonly utilized detergent-based decellularization approach in human autopsy lungs resulted in disproportionate losses of GAGs with depletion of chondroitin sulfate/dermatan sulfate (CS/DS) > heparan sulfate (HS) > hyaluronic acid (HA). Specific changes in disaccharide composition of remaining GAGs were observed with disproportionate loss of NS and NS2S for HS groups and of 4S for CS/DS groups. No significant influence of smoking history, sex, time to autopsy, or age was observed in native vs. decellularized lungs. Notably, surface plasmon resonance demonstrated that GAGs remaining in decellularized lungs were unable to bind key matrix-associated growth factors FGF2, HGF, and TGFβ1. Growth of lung epithelial, pulmonary vascular, and stromal cells cultured on the surface of or embedded within gels derived from decellularized human lungs was differentially and combinatorially enhanced by replenishing specific GAGs and FGF2, HGF, and TGFβ1. In summary, lung decellularization results in loss and/or dysfunction of specific GAGs or side chains significantly affecting matrix-associated growth factor binding and lung cell metabolism. GAG and matrix-associated growth factor replenishment thus needs to be incorporated into schemes for investigations utilizing gels and other materials produced from decellularized human lungs.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
Keyword
- Lung
- Decellularization
- Glycosaminoglycan
- Matrix-associated growth factors
- COPD
- extracellular matrix gel
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Uhl, Franziska
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Zhang, Fuming
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Pouliot, Robert
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Uriarte, Juan Jo ...
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Rolandsson Enes, ...
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Han, Xiaorui
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show more...
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Ouyang, Yilan
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Xia, Ke
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Westergren-Thors ...
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Malmström, Ander ...
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Hallgren, Oskar
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Linhardt, Robert ...
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Weiss, Daniel
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Biomaterials Sci ...
- Articles in the publication
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Acta Biomaterial ...
- By the university
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Lund University