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Superporous agarose...
Superporous agarose as an affinity chromatography support
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- Gustavsson, Per-Erik (författare)
- Lund University,Lunds universitet,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Mosbach, Klaus (författare)
- Lund University,Lunds universitet,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Nilsson, Kjell (författare)
- Percell Biolytica AB
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- Larsson, Per-Olof (författare)
- Lund University,Lunds universitet,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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(creator_code:org_t)
- 1997
- 1997
- Engelska 7 s.
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Ingår i: Journal of chromatography. A. - 0021-9673. ; 776:2, s. 197-203
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Superporous agarose beads were used as an affinity support in column chromatography. These beads characteristically possess two sets of pores, normal diffusion pores and flow pores, so-called superpores. The superpores, whose diameter is a substantial fraction of the particle diameter (i.e. 1/3 to 1/10 of the particle diameter), allow part of the chromatographic flow to pass through each individual bead. Consequently, significant improvement in mass transfer is observed in superporous beads as compared with homogeneous beads, especially at high flow-rates [Gustavsson and Larsson, J. Chromatogr. A, 734 (1996) 231–240.]Superporous agarose beads and homogeneous agarose beads were each derivatized with two types of affinity ligands. A NAD+ analogue was used for the purification of bovine lactate dehydrogenase and protein A was used for the adsorption of rabbit IgG. The performances of superporous beads and homogeneous beads were compared. Superporous bead columns derivatized with protein A and NAD+ analogue could be operated 5 times and 3 times, respectively, as fast as corresponding homogeneous bead columns.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Kemiteknik -- Polymerteknologi (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Chemical Engineering -- Polymer Technologies (hsv//eng)
- NATURVETENSKAP -- Kemi -- Materialkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Materials Chemistry (hsv//eng)
Nyckelord
- Agarose, superporous
- Affinity
- Stationary phases, LC
- Flow pores
- Proteins
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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