Sökning: id:"swepub:oai:lup.lub.lu.se:fa83cbee-9c50-43a1-92d6-e36db1bada1d" >
Lipopolysaccharide ...
Lipopolysaccharide induces cell death in cultured porcine myenteric neurons.
-
Arciszewski, Marcin (författare)
-
- Pierzynowski, Stefan (författare)
- Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Functional zoology,Department of Biology,Faculty of Science
-
- Ekblad, Eva (författare)
- Lund University,Lunds universitet,Neurogastroenterologi,Forskargrupper vid Lunds universitet,Neurogastroenterology,Lund University Research Groups
-
(creator_code:org_t)
- Springer Science and Business Media LLC, 2005
- 2005
- Engelska.
-
Ingår i: Digestive Diseases and Sciences. - : Springer Science and Business Media LLC. - 1573-2568 .- 0163-2116. ; 50:9, s. 1661-1668
- Relaterad länk:
-
https://portal.resea... (primary) (free)
-
visa fler...
-
http://www.ncbi.nlm....
-
http://dx.doi.org/10... (free)
-
https://lup.lub.lu.s...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Enteric bacteria execute, via lipopolysaccharide (LPS), a pathogenic role in intestinal inflammation. The effects of LPS on survival and neurotransmitter expression in cultured porcine myenteric neurons were investigated. Myenteric neurons were isolated and cultured for 6 days in medium, in LPS (100 ng/ml) with or without α-ketoglutarate or the nitric oxide synthase (NOS) inhibitor L-NAME, in α-ketoglutarate or in the NO donor SNAP. Neuronal survival and expression of vasoactive intestinal peptide (VIP) and NOS were evaluated by immunocytochemistry. Addition of LPS significantly decreased neuronal survival; only 40% survived, compared to controls run in parallel. The LPS-induced neurotoxic effect was not counteracted by the simultaneous presence of α-ketoglutarate or L-NAME. Either SNAP or α-ketoglutarate influenced neuronal survival. Culturing, particularly in the presence of LPS, markedly increased the proportion of VIP-immunoreactive neurons; NOS-immunoreactive neurons were unchanged. The reported LPS-induced neurotoxicity indicates loss of enteric neurons as a consequence of intestinal inflammation.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Gastroenterologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Gastroenterology and Hepatology (hsv//eng)
Nyckelord
- Myenteric Plexus: cytology
- Animals
- Cell Culture Techniques
- Cell Death
- Cell Survival
- Female
- Immunohistochemistry
- Inflammation
- Intestinal Diseases: etiology
- Intestinal Diseases: immunology
- Intestinal Diseases: microbiology
- Lipopolysaccharides: pharmacology
- Male
- Myenteric Plexus: pathology
- Neurons: drug effects
- Neurons: physiology
- Neurotransmitters: biosynthesis
- Research Support
- Non-U.S. Gov't
- Swine
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas