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Multisite tyrosine ...
Multisite tyrosine phosphorylation of the N-terminus of Mint1/X11α by Src kinase regulates the trafficking of amyloid precursor protein
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- Dunning, Christopher J R (författare)
- Lund University,Lunds universitet,Klinisk minnesforskning,Forskargrupper vid Lunds universitet,Clinical Memory Research,Lund University Research Groups
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- Black, Hannah L. (författare)
- University of York
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- Andrews, Katie L. (författare)
- University of York
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- Davenport, Elizabeth C. (författare)
- University of York
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- Conboy, Michael (författare)
- University of York
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- Chawla, Sangeeta (författare)
- University of York
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- Dowle, Adam A. (författare)
- University of York
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- Ashford, David (författare)
- University of York
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- Thomas, Jerry R. (författare)
- University of York
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- Evans, Gareth J O (författare)
- University of York
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(creator_code:org_t)
- 2016-03-01
- 2016
- Engelska 10 s.
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Ingår i: Journal of Neurochemistry. - : Wiley. - 0022-3042. ; 137:4, s. 518-527
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://onlinelibrar...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Mint/X11 is one of the four neuronal trafficking adaptors that interact with amyloid precursor protein (APP) and are linked with its cleavage to generate β-amyloid peptide, a key player in the pathology of Alzheimer's disease. How APP switches between adaptors at different stages of the secretory pathway is poorly understood. Here, we show that tyrosine phosphorylation of Mint1 regulates the destination of APP. A canonical SH2-binding motif (202YEEI) was identified in the N-terminus of Mint1 that is phosphorylated on tyrosine by C-Src and recruits the active kinase for sequential phosphorylation of further tyrosines (Y191 and Y187). A single Y202F mutation in the Mint1 N-terminus inhibits C-Src binding and tyrosine phosphorylation. Previous studies observed that co-expression of wild-type Mint1 and APP causes accumulation of APP in the trans-Golgi. Unphosphorylatable Mint1 (Y202F) or pharmacological inhibition of Src reduced the accumulation of APP in the trans-Golgi of heterologous cells. A similar result was observed in cultured rat hippocampal neurons where Mint1(Y202F) permitted the trafficking of APP to more distal neurites than the wild-type protein. These data underline the importance of the tyrosine phosphorylation of Mint1 as a critical switch for determining the destination of APP.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Nyckelord
- amyloid precursor protein
- intracellular trafficking
- Mint1
- protein phosphorylation
- Src
- tyrosine kinase
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Dunning, Christo ...
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Black, Hannah L.
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Andrews, Katie L ...
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Davenport, Eliza ...
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Conboy, Michael
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Chawla, Sangeeta
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visa fler...
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Dowle, Adam A.
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Ashford, David
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Thomas, Jerry R.
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Evans, Gareth J ...
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Neurovetenskaper
- Artiklar i publikationen
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Journal of Neuro ...
- Av lärosätet
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Lunds universitet