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Systems pathology b...
Systems pathology by multiplexed immunohistochemistry and whole-slide digital image analysis
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Blom, S (författare)
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Paavolainen, L (författare)
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Bychkov, D (författare)
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Turkki, R (författare)
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Maki-Teeri, P (författare)
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Hemmes, A (författare)
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Valimaki, K (författare)
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- Lundin, J (författare)
- Karolinska Institutet
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- Kallioniemi, O (författare)
- Karolinska Institutet
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Pellinen, T (författare)
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(creator_code:org_t)
- 2017-11-14
- 2017
- Engelska.
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Ingår i: Scientific reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 7:1, s. 15580-
- Relaterad länk:
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https://www.nature.c...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The paradigm of molecular histopathology is shifting from a single-marker immunohistochemistry towards multiplexed detection of markers to better understand the complex pathological processes. However, there are no systems allowing multiplexed IHC (mIHC) with high-resolution whole-slide tissue imaging and analysis, yet providing feasible throughput for routine use. We present an mIHC platform combining fluorescent and chromogenic staining with automated whole-slide imaging and integrated whole-slide image analysis, enabling simultaneous detection of six protein markers and nuclei, and automatic quantification and classification of hundreds of thousands of cells in situ in formalin-fixed paraffin-embedded tissues. In the first proof-of-concept, we detected immune cells at cell-level resolution (n = 128,894 cells) in human prostate cancer, and analysed T cell subpopulations in different tumour compartments (epithelium vs. stroma). In the second proof-of-concept, we demonstrated an automatic classification of epithelial cell populations (n = 83,558) and glands (benign vs. cancer) in prostate cancer with simultaneous analysis of androgen receptor (AR) and alpha-methylacyl-CoA (AMACR) expression at cell-level resolution. We conclude that the open-source combination of 8-plex mIHC detection, whole-slide image acquisition and analysis provides a robust tool allowing quantitative, spatially resolved whole-slide tissue cytometry directly in formalin-fixed human tumour tissues for improved characterization of histology and the tumour microenvironment.
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- Av författaren/redakt...
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Blom, S
-
Paavolainen, L
-
Bychkov, D
-
Turkki, R
-
Maki-Teeri, P
-
Hemmes, A
-
visa fler...
-
Valimaki, K
-
Lundin, J
-
Kallioniemi, O
-
Pellinen, T
-
visa färre...
- Artiklar i publikationen
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Scientific repor ...
- Av lärosätet
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Karolinska Institutet