Phenalen-1-One-Mediated Antimicrobial Photodynamic Therapy and Chlorhexidine Applied to a Novel Caries Biofilm Model

• Antimicrobial photodynamic therapy (aPDT) may be useful as a supportive antimicrobial measure for caries-active subjects. In this study, the antimicrobial efficacy of aPDT with a phenalen-1-one photosensitizer was evaluated in a novel in vitro biofilm model comprising <i>Actinomyces naeslundii</i>, <i>Actinomyces odontolyticus</i>, and <i>Streptococcus mutans</i> and was compared to chlorhexidine. The proposed biofilm model allows high-throughput screening for antimicrobial efficacy while exhibiting a differentiated response to different antimicrobial approaches. While chlorhexidine 0.2% showed a reduction of ≈4 log₁₀ for all species, aPDT led to a more pronounced reduction of <i>S. mutans</i> (2.8 log₁₀) than of <i>Actinomyces</i> spp. (1.2 or 1.3 log₁₀). A similar effect was also observed in monospecies biofilms. Therefore, aPDT may be more effective against <i>S. mutans</i> than against <i>Actinomyces</i> spp. when in biofilms, and this antimicrobial approach merits further investigations.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Odontologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Dentistry (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Microbiology in the medical area (hsv//eng)

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