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Structure and funct...
Structure and function analysis of the C. elegans aminophospholipid translocase TAT-1
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Chen, YZ (författare)
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- Kloditz, K (författare)
- Karolinska Institutet
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Lee, ES (författare)
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Nguyen, DP (författare)
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Yuan, Q (författare)
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Johnson, J (författare)
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Lee-yow, Y (författare)
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Hall, A (författare)
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Mitani, S (författare)
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Xia, NS (författare)
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- Fadeel, B (författare)
- Karolinska Institutet
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Xue, D (författare)
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(creator_code:org_t)
- 2019-01-01
- 2019
- Engelska.
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Ingår i: Journal of cell science. - : The Company of Biologists. - 1477-9137 .- 0021-9533. ; 132:5
- Relaterad länk:
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https://jcs.biologis...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- The C. elegans aminophospholipid translocase TAT–1 maintains phosphatidylserine (PS) asymmetry in the plasma membrane and regulates endocytic transport. Despite these important functions, the structure-function relationship of this protein is poorly understood. Taking advantage of the tat-1 mutations identified by the C. elegans million mutation project, we investigated the effects of 16 single amino-acid substitutions on the two functions of the TAT–1 protein. Two substitutions that alter a highly conserved PISL motif in the fourth transmembrane domain and a highly conserved DKTGT phosphorylation motif, respectively, disrupt both functions of TAT-1, leading to a vesicular gut defect and ectopic PS exposure on cell surface, whereas most other substitutions across the TAT-1 protein, often predicted to be deleterious by bioinformatics programs, do not affect the functions of TAT-1. These results provide in vivo evidence for the importance of the PISL and DKTGT motifs in P4–type adenosine triphosphatases (ATPases) and improve our understanding of the structure-function relationship of TAT-1. Our study also provides an example of how the C. elegans million mutation project helps decipher the structure, functions, and mechanisms of action of important genes.
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- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Chen, YZ
-
Kloditz, K
-
Lee, ES
-
Nguyen, DP
-
Yuan, Q
-
Johnson, J
-
visa fler...
-
Lee-yow, Y
-
Hall, A
-
Mitani, S
-
Xia, NS
-
Fadeel, B
-
Xue, D
-
visa färre...
- Artiklar i publikationen
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Journal of cell ...
- Av lärosätet
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Karolinska Institutet