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Frequent and Durabl...
Frequent and Durable Anti-HIV Envelope VIV2 IgG Responses Induced by HIV-1 DNA Priming and HIV-MVA Boosting in Healthy Tanzanian Volunteers
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Joachim, A (författare)
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- Msafiri, F (författare)
- Karolinska Institutet
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Onkar, S (författare)
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Munseri, P (författare)
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Aboud, S (författare)
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Lyamuya, EF (författare)
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Bakari, M (författare)
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Billings, E (författare)
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Robb, ML (författare)
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- Wahren, B (författare)
- Karolinska Institutet
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Mhalu, FS (författare)
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Sandstrom, E (författare)
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Rao, M (författare)
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- Nilsson, C (författare)
- Karolinska Institutet
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- Biberfeld, G (författare)
- Karolinska Institutet
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(creator_code:org_t)
- 2020-11-13
- 2020
- Engelska.
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Ingår i: Vaccines. - : MDPI AG. - 2076-393X. ; 8:4
- Relaterad länk:
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https://www.mdpi.com...
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http://kipublication...
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https://doi.org/10.3...
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Abstract
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- We evaluated antibody responses to the human immunodeficiency virus (HIV) envelope variable regions 1 and 2 (V1V2) in 29 vaccinees who had received three HIV-1 DNA immunizations and two HIV-modified vaccinia virus Ankara (MVA) boosts in the phase I/II HIVIS03 vaccine trial. Twenty vaccinees received a third HIV-MVA boost after three years in the HIVIS06 trial. IgG and IgG antibody subclasses to gp70V1V2 proteins of HIV-1 A244, CN54, Consensus C, and Case A2 were analysed using an enzyme-linked immunosorbent assay (ELISA). Cyclic V2 peptides of A244, Consensus C, and MN were used in a surface plasmon resonance (SPR) assay. Four weeks after the second HIV-MVA, anti-V1V2 IgG antibodies to A244 were detected in 97% of HIVIS03 vaccinees, in 75% three years later, and in 95% after the third HIV-MVA. Anti-CN54 V1V2 IgG was detectable in 48% four weeks after the second HIV-MVA. The SPR data supported the findings. The IgG response was predominantly IgG1. Four weeks after the second HIV-MVA, 85% of vaccinees had IgG1 antibodies to V1V2 A244, which persisted in 25% for three-years. IgG3 and IgG4 antibodies to V1V2 A244 were rare. In conclusion, the HIV-DNA/MVA vaccine regimen induced durable V1V2 IgG antibody responses in a high proportion of vaccinees.
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- art (ämneskategori)
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Vaccines
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Joachim, A
-
Msafiri, F
-
Onkar, S
-
Munseri, P
-
Aboud, S
-
Lyamuya, EF
-
visa fler...
-
Bakari, M
-
Billings, E
-
Robb, ML
-
Wahren, B
-
Mhalu, FS
-
Sandstrom, E
-
Rao, M
-
Nilsson, C
-
Biberfeld, G
-
visa färre...
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Vaccines
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Karolinska Institutet