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Comparison of Cytom...
Comparison of Cytomegalovirus-Specific Immune Cell Response to Proteins versus Peptides Using an IFN-γ ELISpot Assay after Hematopoietic Stem Cell Transplantation
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Wagner-Drouet, E (författare)
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Teschner, D (författare)
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Wolschke, C (författare)
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Schafer-Eckart, K (författare)
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Gartner, J (författare)
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- Mielke, S (författare)
- Karolinska Institutet
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Schreder, M (författare)
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Kobbe, G (författare)
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Hilgendorf, I (författare)
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Klein, S (författare)
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Verbeek, M (författare)
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Ditschkowski, M (författare)
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Koch, M (författare)
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Lindemann, M (författare)
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Schmidt, T (författare)
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Rascle, A (författare)
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Barabas, S (författare)
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Deml, L (författare)
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Wagner, R (författare)
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Wolff, D (författare)
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(creator_code:org_t)
- 2021-02-15
- 2021
- Engelska.
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Ingår i: Diagnostics (Basel, Switzerland). - : MDPI AG. - 2075-4418. ; 11:2
- Relaterad länk:
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https://www.mdpi.com...
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http://kipublication...
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https://doi.org/10.3...
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Abstract
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- Cytomegalovirus (CMV) infection is a major cause of morbidity and mortality following hematopoietic stem cell transplantation (HSCT). Measuring CMV-specific cellular immunity may improve the risk stratification and management of patients. IFN-γ ELISpot assays, based on the stimulation of peripheral blood mononuclear cells with CMV pp65 and IE-1 proteins or peptides, have been validated in clinical settings. However, it remains unclear to which extend the T-cell response to synthetic peptides reflect that mediated by full-length proteins processed by antigen-presenting cells. We compared the stimulating ability of pp65 and IE-1 proteins and corresponding overlapping peptides in 16 HSCT recipients using a standardized IFN-γ ELISpot assay. Paired qualitative test results showed an overall 74.4% concordance. Discordant results were mainly due to low-response tests, with one exception. One patient with early CMV reactivation and graft-versus-host disease, sustained CMV DNAemia and high CD8+ counts showed successive negative protein-based ELISpot results but a high and sustained response to IE-1 peptides. Our results suggest that the response to exogenous proteins, which involves their uptake and processing by antigen-presenting cells, more closely reflects the physiological response to CMV infection, while the response to exogenous peptides may lead to artificial in vitro T-cell responses, especially in strongly immunosuppressed patients.
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- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Wagner-Drouet, E
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Teschner, D
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Wolschke, C
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Schafer-Eckart, ...
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Gartner, J
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Mielke, S
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visa fler...
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Schreder, M
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Kobbe, G
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Hilgendorf, I
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Klein, S
-
Verbeek, M
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Ditschkowski, M
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Koch, M
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Lindemann, M
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Schmidt, T
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Rascle, A
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Barabas, S
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Deml, L
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Wagner, R
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Wolff, D
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visa färre...
- Artiklar i publikationen
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Diagnostics (Bas ...
- Av lärosätet
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Karolinska Institutet