Sökning: id:"swepub:oai:prod.swepub.kib.ki.se:146693920" >
Short-term IL-15 pr...
Short-term IL-15 priming leaves a long-lasting signalling imprint in mouse NK cells independently of a metabolic switch
-
Luu, TT (författare)
-
Schmied, L (författare)
-
Nguyen, NA (författare)
-
visa fler...
-
Wiel, C (författare)
-
- Meinke, S (författare)
- Karolinska Institutet
-
- Mohammad, DK (författare)
- Karolinska Institutet
-
- Bergo, M (författare)
- Karolinska Institutet
-
- Alici, E (författare)
- Karolinska Institutet
-
- Kadri, N (författare)
- Karolinska Institutet
-
Ganesan, S (författare)
-
- Hoglund, P (författare)
- Karolinska Institutet
-
visa färre...
-
(creator_code:org_t)
- 2021-02-16
- 2021
- Engelska.
-
Ingår i: Life science alliance. - : Life Science Alliance, LLC. - 2575-1077. ; 4:4
- Relaterad länk:
-
https://www.life-sci...
-
visa fler...
-
http://kipublication...
-
https://doi.org/10.2...
-
visa färre...
Abstract
Ämnesord
Stäng
- IL-15 priming of NK cells is a broadly accepted concept, but the dynamics and underlying molecular mechanisms remain poorly understood. We show that as little as 5 min of IL-15 treatment in vitro, followed by removal of excess cytokines, results in a long-lasting, but reversible, augmentation of NK cell responsiveness upon activating receptor cross-linking. In contrast to long-term stimulation, improved NK cell function after short-term IL-15 priming was not associated with enhanced metabolism but was based on the increased steady-state phosphorylation level of signalling molecules downstream of activating receptors. Inhibition of JAK3 eliminated this priming effect, suggesting a cross talk between the IL-15 receptor and ITAM-dependent activating receptors. Increased signalling molecule phosphorylation levels, calcium flux, and IFN-γ secretion lasted for up to 3 h after IL-15 stimulation before returning to baseline. We conclude that IL-15 rapidly and reversibly primes NK cell function by modulating activating receptor signalling. Our findings suggest a mechanism by which NK cell reactivity can potentially be maintained in vivo based on only brief encounters with IL-15 trans-presenting cells.
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Luu, TT
-
Schmied, L
-
Nguyen, NA
-
Wiel, C
-
Meinke, S
-
Mohammad, DK
-
visa fler...
-
Bergo, M
-
Alici, E
-
Kadri, N
-
Ganesan, S
-
Hoglund, P
-
visa färre...
- Artiklar i publikationen
-
Life science all ...
- Av lärosätet
-
Karolinska Institutet