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Human Cytomegalovir...
Human Cytomegalovirus Reduces Endothelin-1 Expression in Both Endothelial and Vascular Smooth Muscle Cells
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- Yaiw, KC (författare)
- Karolinska Institutet
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Mohammad, AA (författare)
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Taher, C (författare)
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Cui, HL (författare)
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Costa, H (författare)
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- Kostopoulou, ON (författare)
- Karolinska Institutet
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Jung, M (författare)
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Assinger, A (författare)
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Wilhelmi, V (författare)
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- Yang, JN (författare)
- Karolinska Institutet
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Straat, K (författare)
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- Rahbar, A (författare)
- Karolinska Institutet
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- Pernow, J (författare)
- Karolinska Institutet
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- Soderberg-Naucler, C (författare)
- Karolinska Institutet
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(creator_code:org_t)
- 2021-05-25
- 2021
- Engelska.
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Ingår i: Microorganisms. - : MDPI AG. - 2076-2607. ; 9:6
- Relaterad länk:
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https://www.mdpi.com...
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http://kipublication...
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https://doi.org/10.3...
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Abstract
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- Human cytomegalovirus (HCMV) is an opportunistic pathogen that has been implicated in the pathogenesis of atherosclerosis. Endothelin-1 (ET-1), a potent vasoconstrictive peptide, is overexpressed and strongly associated with many vasculopathies. The main objective of this study was to investigate whether HCMV could affect ET-1 production. As such, both endothelial and smooth muscle cells, two primary cell types involved in the pathogenesis of atherosclerosis, were infected with HCMV in vitro and ET-1 mRNA and proteins were assessed by quantitative PCR assay, immunofluorescence staining and ELISA. HCMV infection significantly decreased ET-1 mRNA and secreted bioactive ET-1 levels from both cell types and promoted accumulation of the ET-1 precursor protein in infected endothelial cells. This was associated with inhibition of expression of the endothelin converting enzyme-1 (ECE-1), which cleaves the ET-1 precursor protein to mature ET-1. Ganciclovir treatment did not prevent the virus suppressive effects on ET-1 expression. Consistent with this observation we identified that the IE2-p86 protein predominantly modulated ET-1 expression. Whether the pronounced effects of HCMV in reducing ET-1 expression in vitro may lead to consequences for regulation of the vascular tone in vivo remains to be proven.
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Yaiw, KC
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Mohammad, AA
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Taher, C
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Cui, HL
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Costa, H
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Kostopoulou, ON
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visa fler...
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Jung, M
-
Assinger, A
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Wilhelmi, V
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Yang, JN
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Straat, K
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Rahbar, A
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Pernow, J
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Soderberg-Naucle ...
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visa färre...
- Artiklar i publikationen
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Microorganisms
- Av lärosätet
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Karolinska Institutet