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Subinhibitory antib...
Subinhibitory antibiotic concentrations promote the horizontal transfer of plasmid-borne resistance genes from Klebsiellae pneumoniae to Escherichia coli
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Ding, ML (författare)
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Ye, Z (författare)
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Liu, L (författare)
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Wang, W (författare)
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Chen, Q (författare)
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Zhang, FY (författare)
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Wang, Y (författare)
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- Sjoling, A (författare)
- Karolinska Institutet
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- Martin-Rodriguez, AJ (författare)
- Karolinska Institutet
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Hu, RJ (författare)
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Chen, WB (författare)
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Zhou, YS (författare)
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visa färre...
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(creator_code:org_t)
- 2022-11-07
- 2022
- Engelska.
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Ingår i: Frontiers in microbiology. - : Frontiers Media SA. - 1664-302X. ; 13, s. 1017092-
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http://kipublication...
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https://doi.org/10.3...
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Abstract
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- Horizontal gene transfer plays an important role in the spread of antibiotic resistance, in which plasmid-mediated conjugation transfer is the most important mechanism. While sub-minimal inhibitory concentrations (sub-MIC) of antibiotics could promote conjugation frequency, the mechanism by which sub-MIC levels of antibiotics affect conjugation frequency is not clear. Here, we used Klebsiella pneumoniae SW1780 carrying the multi-drug resistance plasmid pSW1780-KPC as the donor strain, to investigate the effects of sub-MICs of meropenem (MEM), ciprofloxacin (CIP), cefotaxime (CTX), and amikacin (AK) on conjugational transfer of pSW1780-KPC from SW1780 to Escherichia coli J53. Our results showed that the transfer frequencies increased significantly by treating SW1780 strain with sub-MIC levels of MEM, CIP, CTX and AK. Transfer frequencies at sub-MIC conditions in a Galleria mellonella were significantly higher than in vitro. To investigate gene expression and metabolic effects, RT-qPCR and LC–MS-based metabolome sequencing were performed. Transcript levels of T4SS genes virB1, virB2, virB4, virB8, and conjugation-related genes traB, traK, traE, and traL were significantly upregulated by exposure to sub-MICs of MEM, CIP, CTX, and AK. Metabolome sequencing revealed nine differentially regulated metabolites. Our findings are an early warning for a wide assessment of the roles of sub-MIC levels of antibiotics in the spread of antibiotic resistance.
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- Av författaren/redakt...
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Ding, ML
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Ye, Z
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Liu, L
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Wang, W
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Chen, Q
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Zhang, FY
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visa fler...
-
Wang, Y
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Sjoling, A
-
Martin-Rodriguez ...
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Hu, RJ
-
Chen, WB
-
Zhou, YS
-
visa färre...
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Frontiers in mic ...
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Karolinska Institutet