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Mucosal and plasma ...
Mucosal and plasma IgA from HIV-1-exposed uninfected individuals inhibit HIV-1 transcytosis across human epithelial cells
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Devito, C (författare)
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- Broliden, K (författare)
- Karolinska Institutet
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Kaul, R (författare)
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Svensson, L (författare)
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Johansen, K (författare)
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Kiama, P (författare)
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Kimani, J (författare)
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Lopalco, L (författare)
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Piconi, S (författare)
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Bwayo, JJ (författare)
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Plummer, F (författare)
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Clerici, M (författare)
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Hinkula, J (författare)
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(creator_code:org_t)
- The American Association of Immunologists, 2000
- 2000
- Engelska.
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Ingår i: Journal of immunology (Baltimore, Md. : 1950). - : The American Association of Immunologists. - 0022-1767 .- 1550-6606. ; 165:9, s. 5170-5176
- Relaterad länk:
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http://www.jimmunol....
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http://kipublication...
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https://doi.org/10.4...
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Abstract
Ämnesord
Stäng
- HIV-1-specific IgA has been described in the genital tract and plasma of HIV-1 highly exposed, persistently seronegative (HEPS) individuals, and IgA from these sites has been shown to neutralize HIV-1. This study examines the ability of IgA isolated from HEPS individuals to inhibit transcytosis across a tight epithelial cell layer. A Transwell system was established to model HIV-1 infection across the human mucosal epithelium. The apical-basolateral transcytosis of primary HIV-1 isolates across this mucosal model was examined in the presence and the absence of IgA isolated from the genital tract, saliva, and plasma of HEPS individuals enrolled in both a sex worker cohort in Nairobi, Kenya, and a discordant couple cohort in Italy. In the absence of IgA, HIV-1 primary isolates were actively transported across the epithelial membrane and were released on the opposite side of the barrier. These transcytosed HIV-1 particles retained their ability to infect human mononuclear cells. However, IgA purified from the mucosa and plasma of HEPS individuals was able to inhibit HIV-1 transcytosis. Inhibition was seen in three of six cervicovaginal fluid samples, five of 10 saliva samples, and three of six plasma samples against at least one of the two primary HIV-1 isolates tested. IgA from low risk, healthy control subjects had no inhibitory effect on HIV-1 transcytosis. The ability of mucosal and plasma IgA to inhibit HIV-1 transcytosis across the mucosal epithelium may represent an important mechanism for protection against the sexual acquisition of HIV-1 infection in HEPS individuals.
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- Av författaren/redakt...
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Devito, C
-
Broliden, K
-
Kaul, R
-
Svensson, L
-
Johansen, K
-
Kiama, P
-
visa fler...
-
Kimani, J
-
Lopalco, L
-
Piconi, S
-
Bwayo, JJ
-
Plummer, F
-
Clerici, M
-
Hinkula, J
-
visa färre...
- Artiklar i publikationen
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Journal of immun ...
- Av lärosätet
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Karolinska Institutet