Sökning: id:"swepub:oai:prod.swepub.kib.ki.se:1945095" >
Intranasal HIV-1-gp...
Intranasal HIV-1-gp160-DNA/gp41 peptide prime-boost immunization regimen in mice results in long-term HIV-1 neutralizing humoral mucosal and systemic immunity
-
Devito, C (författare)
-
Zuber, B (författare)
-
Schroder, U (författare)
-
visa fler...
-
Benthin, R (författare)
-
Okuda, K (författare)
-
- Broliden, K (författare)
- Karolinska Institutet
-
- Wahren, B (författare)
- Karolinska Institutet
-
Hinkula, J (författare)
-
visa färre...
-
(creator_code:org_t)
- The American Association of Immunologists, 2004
- 2004
- Engelska.
-
Ingår i: Journal of immunology (Baltimore, Md. : 1950). - : The American Association of Immunologists. - 0022-1767 .- 1550-6606. ; 173:11, s. 7078-7089
- Relaterad länk:
-
http://www.jimmunol....
-
visa fler...
-
http://kipublication...
-
https://doi.org/10.4...
-
visa färre...
Abstract
Ämnesord
Stäng
- An intranasal DNA vaccine prime followed by a gp41 peptide booster immunization was compared with gp41 peptide and control immunizations. Serum HIV-1-specific IgG and IgA as well as IgA in feces and vaginal and lung secretions were detected after immunizations. Long-term humoral immunity was studied for up to 12 mo after the booster immunization by testing the presence of HIV-1 gp41- and CCR5-specific Abs and IgG/IgA-secreting B lymphocytes in spleen and regional lymph nodes in immunized mice. A long-term IgA-specific response in the intestines, vagina, and lungs was obtained in addition to a systemic immune response. Mice immunized only with gp41 peptides and L3 adjuvant developed a long-term gp41-specific serum IgG response systemically, although over a shorter period (1–9 mo), and long-term mucosal gp41-specific IgA immunity. HIV-1-neutralizing serum Abs were induced that were still present 12 mo after booster immunization. HIV-1 SF2-neutralizing fecal and lung IgA was detectable only in the DNA-primed mouse groups. Intranasal DNA prime followed by one peptide/L3 adjuvant booster immunization, but not a peptide prime followed by a DNA booster, was able to induce B cell memory and HIV-1-neutralizing Abs for at least half of a mouse’s life span.
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas