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Kit ligand and c-Ki...
Kit ligand and c-Kit are expressed during early human ovarian follicular development and their interaction is required for the survival of follicles in long-term culture
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Carlsson, IB (författare)
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Laitinen, MPE (författare)
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Scott, JE (författare)
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Louhio, H (författare)
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Velentzis, L (författare)
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Tuuri, T (författare)
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Aaltonen, J (författare)
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Ritvos, O (författare)
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Winston, RML (författare)
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- Hovatta, O (författare)
- Karolinska Institutet
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(creator_code:org_t)
- Bioscientifica, 2006
- 2006
- Engelska.
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Ingår i: Reproduction (Cambridge, England). - : Bioscientifica. - 1470-1626 .- 1741-7899. ; 131:4, s. 641-649
- Relaterad länk:
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https://rep.bioscien...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The receptor tyrosine c-Kit and its cognate ligand, c-Kit ligand (KL, stem cell factor, SCF), are involved in ovarian follicular development in several animal species. We studied the expression of KL and c-Kit usingin situhybridization and immunohistochemistry in donated human ovarian cortical tissue. The KL transcripts were expressed in granulosa cells of primary follicles, whereas the expression of c-Kit was confined to the oocyte and granulosa cells in primary and secondary follicles. We employed an ovarian organ culture using firstly serum-containing and then serum-free medium to study the effects of KL and an anti-c-Kit antibody, ACK2, on the development and survival of ovarian folliclesin vitro. Culture of ovarian cortical slices for 7 days resulted in a 37% increase in the number of primary follicles and a 6% increase in secondary follicles. The proportion of viable follicles decreased in all cultures. The addition of KL (1, 10 and 100 ng/ml) into the culture media did not affect the developmental stages of the follicles or the proportion of atretic follicles. Inclusion of ACK2 (800 ng/ml) in the culture medium significantly increased the proportion of atretic follicles on days 7 (49 vs 28% in control cultures) and 14 (62 vs 38%) of culture. In conclusion, c-Kit and KL are expressed in human ovaries during follicular development. Blocking the c-Kit receptor induces follicular atresia. The KL/c-Kit signaling system is likely to control the survival of human ovarian follicles during early follicular development.
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Carlsson, IB
-
Laitinen, MPE
-
Scott, JE
-
Louhio, H
-
Velentzis, L
-
Tuuri, T
-
visa fler...
-
Aaltonen, J
-
Ritvos, O
-
Winston, RML
-
Hovatta, O
-
visa färre...
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Reproduction (Ca ...
- Av lärosätet
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Karolinska Institutet