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Protein-Containing ...
Protein-Containing Lipid Bilayers Intercalated with Size-Matched Mesoporous Silica Thin Films
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- Isaksson, Simon, 1988 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Chalmers, Dept Chem & Chem Engn, SE-41296 Gothenburg, Sweden.
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- Watkins, E. B. (author)
- Los Alamos National Laboratory,Los Alamos Natl Lab, Mat Phys & Applicat Div, MPA 11, Los Alamos, NM 87545 USA.
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- Browning, Kathryn L. (author)
- Uppsala universitet,Institutionen för farmaci,Uppsala University
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- Lind, Tania Kjellerup (author)
- Malmö högskola,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces
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- Cárdenas, Marité (author)
- Malmö högskola,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces
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- Hedfalk, Kristina, 1969 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Höök, Fredrik, 1966 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Chalmers, Dept Appl Phys, SE-41296 Gothenburg, Sweden.
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- Andersson, Martin, 1974 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Chalmers, Dept Chem & Chem Engn, SE-41296 Gothenburg, Sweden.
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Chalmers tekniska högskola Chalmers, Dept Chem & Chem Engn, SE-41296 Gothenburg, Sweden (creator_code:org_t)
- 2016-11-29
- 2017
- English.
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In: Nano Letters. - : American Chemical Society (ACS). - 1530-6992 .- 1530-6984. ; 17:1, s. 476-485
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Abstract
Subject headings
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- Proteins are key components in a multitude of biological processes, of which the functions carried out by transmembrane (membrane-spanning) proteins are especially demanding for investigations. This is because this class of protein needs to be incorporated into a lipid bilayer representing its native environment, and in addition, many experimental conditions also require a solid support for stabilization and analytical purposes. The solid support substrate may, however, limit the protein functionality due to protein material interactions and a lack of physical space. We have in this work tailored the pore size and pore ordering of a mesoporous silica thin film to match the native cell-membrane arrangement of the transmembrane protein human aquaporin 4 (hAQP4). Using neutron reflectivity (NR), we provide evidence of how substrate pores host the bulky water-soluble domain of hAQP4, which is shown to extend 7.2 nm into the pores of the substrate. Complementary surface analytical tools, including quartz crystal microbalance with dissipation monitoring (QCM-D) and fluorescence microscopy, revealed successful protein-containing supported lipid bilayer (pSLB) formation on mesoporous silica substrates, whereas pSLB formation was hampered on nonporous silica. Additionally, electron microscopy (TEM and SEM), light scattering (DLS and stopped-flow), and small-angle X-ray scattering (SAXS) were employed to provide a comprehensive characterization of this novel hybrid organic-inorganic interface, the tailoring of which is likely to be generally applicable to improve the function and stability of a broad range of membrane proteins containing water-soluble domains.
Subject headings
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Kemiteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Chemical Engineering (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- NATURVETENSKAP -- Fysik -- Den kondenserade materiens fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Condensed Matter Physics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Materialkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Materials Chemistry (hsv//eng)
Keyword
- Membrane protein
- Lipid bilayer
- membranes
- titania
- Aquaporin
- conductance
- angstrom
- Neutron reflectivity
- Liposome
- Silica
- vesicle fusion
- channel
- Aquaporin; Lipid bilayer; Liposome; Membrane protein; Neutron reflectivity; Silica
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Isaksson, Simon, ...
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Watkins, E. B.
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Browning, Kathry ...
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Lind, Tania Kjel ...
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Cárdenas, Marité
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Hedfalk, Kristin ...
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Höök, Fredrik, 1 ...
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Andersson, Marti ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Chemical Enginee ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Medical Biotechn ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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and Condensed Matter ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Materials Chemis ...
- Articles in the publication
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Nano Letters
- By the university
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Chalmers University of Technology
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Uppsala University
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Malmö University
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University of Gothenburg