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Diamond cubic phase...
Diamond cubic phase of monoolein and water as an amphiphilic matrix for electrophoresis of oligonucleotides
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- Carlsson, Nils, 1978 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Winge, AS, 1966 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Engström, Sven, 1951 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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visa fler...
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- Åkerman, Björn, 1957 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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visa färre...
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(creator_code:org_t)
- 2005
- 2005
- Engelska.
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Ingår i: JOURNAL OF PHYSICAL CHEMISTRY B. ; 109:39, s. 18628-18636
- Relaterad länk:
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https://research.cha...
Abstract
Ämnesord
Stäng
- We used a cubic liquid crystal formed by the nonionic monoglyceride monoolein and water as a porous matrix for the electrophoresis of oligonucleotides. The diamond cubic phase is thermodynamically stable when in contact with a water-rich phase, which we exploit to run the electrophoresis in the useful submarine mode. Oligonucleotides are separated according to size and secondary structure by migration through the space-filling aqueous nanometer pores of the regular liquid crystal, but the comparatively slow migration means the cubic phase will not be a replacement for the conventional DNA gels. However, our demonstration that the cubic phase can be used in submarine electrophoresis opens up the possibility for a new matrix for electrophoresis of amphiphilic molecules. From this perspective, the results on the oligonucleotides show that water-soluble particles of nanometer size, typical for the hydrophilic parts of membrane-bound proteins, may be a useful separation motif. A charged contamination in the commercial sample of monoolein, most likely oleic acid that arises from its hydrolysis, restricts useful buffer conditions to a pH below 5.6.
Ämnesord
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
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