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CRISPR/Cas9 Based G...
CRISPR/Cas9 Based Genome Editing of Penicillium chrysogenum
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- Pohl, C. (författare)
- Rijksuniversiteit Groningen,University of Groningen
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- Kiel, J. A. (författare)
- Rijksuniversiteit Groningen,University of Groningen
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- Driessen, A. J. (författare)
- Rijksuniversiteit Groningen,University of Groningen
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- Bovenberg, R. A. (författare)
- DSM Biotechnology Centre,Rijksuniversiteit Groningen,University of Groningen
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- Nygård, Yvonne, 1986 (författare)
- Rijksuniversiteit Groningen,University of Groningen
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(creator_code:org_t)
- 2016-05-03
- 2016
- Engelska.
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Ingår i: ACS Synthetic Biology. - : American Chemical Society (ACS). - 2161-5063. ; 5:7, s. 754-64
- Relaterad länk:
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https://pure.rug.nl/...
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https://research.cha...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- CRISPR/Cas9 based systems have emerged as versatile platforms for precision genome editing in a wide range of organisms. Here we have developed powerful CRISPR/Cas9 tools for marker-based and marker-free genome modifications in Penicillium chrysogenum, a model filamentous fungus and industrially relevant cell factory. The developed CRISPR/Cas9 toolbox is highly flexible and allows editing of new targets with minimal cloning efforts. The Cas9 protein and the sgRNA can be either delivered during transformation, as preassembled CRISPR-Cas9 ribonucleoproteins (RNPs) or expressed from an AMA1 based plasmid within the cell. The direct delivery of the Cas9 protein with in vitro synthesized sgRNA to the cells allows for a transient method for genome engineering that may rapidly be applicable for other filamentous fungi. The expression of Cas9 from an AMA1 based vector was shown to be highly efficient for marker-free gene deletions.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Annan industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Other Industrial Biotechnology (hsv//eng)
Nyckelord
- Oligonucleotides/genetics
- *genome editing
- RNA
- Genetic Markers
- Fungal
- Genome
- *CRISPR-Cas Systems
- *marker-free gene deletion
- Penicillium chrysogenum/*genetics
- Genetic Vectors
- *Penicillium chrysogenum
- Gene Editing/*methods
- Gene Deletion
- *Rnp
- Bacterial Proteins/genetics
- *CRISPR/Cas9
- Gene Targeting/methods
- Guide
- Endonucleases/genetics
- DNA Repair
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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