Kinetic and thermodynamic characterization of single-mismatch discrimination using single-molecule imaging

• A single-molecule detection setup based on total internal reflection fluorescence (TIRF) microscopy has been used to investigate association and dissociation kinetics of unlabeled 30mer DNA strands. Single-molecule sensitivity was accomplished by letting unlabeled DNA target strands mediate the binding of DNA-modified and fluorescently labeled liposomes to a DNA-modified surface. The liposomes, acting as signal enhancer elements, enabled the number of binding events as well as the residence time for high affinity binders (K-d

Ämnesord

NATURVETENSKAP  -- Biologi -- Biofysik (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biophysics (hsv//eng)

Nyckelord

hybridization

dissociation

sequence

density oligonucleotide arrays

base-pairs

surface-plasmon resonance

dna

nanoparticles

microarrays

nucleotide polymorphisms

nucleic-acids

Publikations- och innehållstyp

art (ämneskategori)

ref (ämneskategori)