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2D-NMR (HSQC) diffe...
2D-NMR (HSQC) difference spectra between specifically C-13-enriched and unenriched protolignin of Ginkgo biloba obtained in the solution state of whole cell wall material
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- Terashima, N. (författare)
- 2-610 Uedayama, Tenpaku, Nagoya, Japan
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- Akiyama, T. (författare)
- USDA ARS U.S. Dairy Forage Research Center,Dairy Forage Research Center, USDA-ARS, Madison,WI, USA
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- Ralph, S. (författare)
- USDA Forest Products Laboratory,Forest Products Laboratory, Madison, WI, USA
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- Evtuguin, D. (författare)
- CICECO/Department of Chemistry, Aveiro University, Aveiro, Portugal
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- Neto, C. P. (författare)
- CICECO/Department of Chemistry, Aveiro University, Aveiro, Portugal
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- Parkås, Jim, 1974 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,Department of Forest Products and Chemical Engineering, Chalmers University of Technology, Göteborg, Sweden; Södra Cell AB, R&D, Väröbacka, Sweden
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- Paulsson, Magnus, 1966 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,Department of Forest Products and Chemical Engineering, Chalmers University of Technology, Göteborg, Sweden; EKA Chemicals AB, Bohus, Sweden
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- Westermark, Ulla (författare)
- Luleå tekniska universitet,Träteknik
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- Ralph, J. (författare)
- University of Wisconsin Madison,Department of Biochemistry and Great Lakes Bioenergy Research Center, University of Wisconsin, Madison, WI, USA (formerly US Dairy Forage Research Center, USDA-ARS, Madison, WI, USA)
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2-610 Uedayama, Tenpaku, Nagoya, Japan USDA ARS US. Dairy Forage Research Center (creator_code:org_t)
- 2009
- 2009
- Engelska.
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Ingår i: Holzforschung. - 1437-434X .- 0018-3830. ; 63:4, s. 379-384
- Relaterad länk:
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http://dx.doi.org/10...
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https://research.cha...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- In the structural analysis of lignins by C-13-NMR, signal overlap limits definitive assignment and accurate intensity measurement. Selective labeling by C-13-enrichment of a specific carbon in lignin enhances its signal intensity in the spectrum. Further enhancement of the specifically labeled carbons can be realized via difference spectra created from the enriched and unenriched samples. Difference 2D C-13-H-1 correlation (HSQC) NMR spectra, derived from the spectra of specifically C-13-enriched lignin model polymers (so-called dehydrogenation polymers) and their unenriched counterparts, take advantage of the enhanced dispersion afforded by both C-13 and H-1 chemical shifts, diminishing the difficulties arising from the signal-overlap problem and aiding in definitive signal assignments. In this research, protolignin in xylem cell walls was specifically C-13-enriched at all of the individual phenylpropanoid side-chain carbons by feeding C-13-enriched coniferins to growing stems of Ginkgo biloba. The whole xylem fractions containing C-13-enriched and unenriched protolignins were dissolved in a mixture of N-methylimidazole and DMSO, and then acetylated. Solution state 2D-NMR (HSQC) spectra of the acetylated whole cell wall were acquired. Difference spectra between the walls containing C-13-enriched and unenriched lignins afforded simplified 2D spectra in which well-separated signals were assigned exclusively to the specifically enriched carbons. This novel NMR technique provides a useful tool for elucidation of entire protolignin in the cell wall of ginkgo xylem.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Annan teknik -- Övrig annan teknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Other Engineering and Technologies -- Other Engineering and Technologies not elsewhere specified (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biomaterial (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bio Materials (hsv//eng)
Nyckelord
- enrichment
- part 1
- lignification
- ginkgo
- side-chain carbons
- dehydrogenation
- coniferin
- nuclear-magnetic-resonance
- 2D-difference NMR spectroscopy
- lignin biosynthesis
- glucosides
- polymer (DHP)
- carbon-13 enrichment
- protolignin
- spruce
- nmr-spectroscopy
- carbon-13-NMR spectroscopy
- Träfysik
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Terashima, N.
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Akiyama, T.
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Ralph, S.
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Evtuguin, D.
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Neto, C. P.
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Parkås, Jim, 197 ...
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visa fler...
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Paulsson, Magnus ...
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Westermark, Ulla
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Ralph, J.
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- Om ämnet
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Annan teknik
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och Övrig annan tekn ...
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Industriell biot ...
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och Biomaterial
- Artiklar i publikationen
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Holzforschung
- Av lärosätet
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Chalmers tekniska högskola
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Luleå tekniska universitet