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Immobilization of b...
Immobilization of bacterial feruloyl esterase on mesoporous silica particles and enhancement of synthetic activity by hydrophobic-modified surface
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- Chong, Sun-Li, 1976 (författare)
- Zhejiang Agriculture and Forestry University,Chalmers tekniska högskola,Chalmers University of Technology
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- Cardoso, Vânia (författare)
- Universidade de Lisboa,University of Lisbon,Nzytech Lda
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- Brás, Joana L.A. (författare)
- Nzytech Lda
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- Zezzi Do Valle Gomes, Milene, 1987 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Fontes, Carlos M.G.A. (författare)
- Nzytech Lda,Universidade de Lisboa,University of Lisbon
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- Olsson, Lisbeth, 1963 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- Elsevier BV, 2019
- 2019
- Engelska.
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Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 293
- Relaterad länk:
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https://research.cha... (primary) (free)
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https://doi.org/10.1...
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- Here, we demonstrated the immobilization of bacterial feruloyl esterase (FAE) from Butyrivibrio sp. XPD2006, Lactobacillus crispatus, Butyrivibrio sp. AE2015, Ruminococcus albus, Cellulosilyticum ruminicola and Clostridium cellulovorans on SBA-15 and their ability to synthesize butyl ferulate (BFA). The BFae2 from Butyrivibrio sp. XPD2006 showed the best catalytic efficiency. High BFA yield was produced when the immobilization of BFae2 took place with a high protein loading and narrow pore sized SBA-15, suggesting alteration of enzyme behavior due to the crowding environment in SBA-15. Grafting of SBA-15 with octyl moieties led to shrinking pore size and resulted in 2.5-fold increment of BFA activity compared to the free enzyme and 70%mol BFA was achieved. The BFae2 encapsulated in hydrophobic-modified SBA-15 endured up to seven reaction cycles while the BFA activity remained above 60%. This is the first report showing the superior performance of hydrophobic-modified surface to entrap FAE to produce fatty phenolic esters.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
Nyckelord
- Protein loading
- Transesterification
- Pore size
- SBA-15
- Butyl ferulate
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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