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Improved production...
Improved production of human hemoglobin in yeast by engineering hemoglobin degradation
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- Ishchuk, Olena, 1980 (författare)
- Chalmers University of Technology
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- Frost, August T. (författare)
- Danmarks Tekniske Universitet,Technical University of Denmark
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- Muniz, Facundo, 1987 (författare)
- Chalmers University of Technology
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- Matsumoto, Saki, 1994 (författare)
- Chalmers University of Technology
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- Laforge, Nathalie, 1996 (författare)
- Chalmers University of Technology
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- Eriksson, Nélida Leiva (författare)
- Lund University,Lunds universitet,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Martinez Ruiz, Jose Luis, 1981 (författare)
- Chalmers University of Technology,Technical University of Denmark
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- Petranovic Nielsen, Dina, 1975 (författare)
- Chalmers University of Technology
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(creator_code:org_t)
- Elsevier BV, 2021
- 2021
- Engelska.
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Ingår i: Metabolic Engineering. - : Elsevier BV. - 1096-7176 .- 1096-7184. ; 66, s. 259-267
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Abstract
Ämnesord
Stäng
- With the increasing demand for blood transfusions, the production of human hemoglobin (Hb) from sustainable sources is increasingly studied. Microbial production is an attractive option, as it may provide a cheap, safe, and reliable source of this protein. To increase the production of human hemoglobin by the yeast Saccharomyces cerevisiae, the degradation of Hb was reduced through several approaches. The deletion of the genes HMX1 (encoding heme oxygenase), VPS10 (encoding receptor for vacuolar proteases), PEP4 (encoding vacuolar proteinase A), ROX1 (encoding heme-dependent repressor of hypoxic genes) and the overexpression of the HEM3 (encoding porphobilinogen deaminase) and the AHSP (encoding human alpha-hemoglobin-stabilizing protein) genes — these changes reduced heme and Hb degradation and improved heme and Hb production. The reduced hemoglobin degradation was validated by a bilirubin biosensor. During glucose fermentation, the engineered strains produced 18% of intracellular Hb relative to the total yeast protein, which is the highest production of human hemoglobin reported in yeast. This increased hemoglobin production was accompanied with an increased oxygen consumption rate and an increased glycerol yield, which (we speculate) is the yeast's response to rebalance its NADH levels under conditions of oxygen limitation and increased protein-production.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Annan industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Other Industrial Biotechnology (hsv//eng)
Nyckelord
- Bilirubin biosensor
- Human hemoglobin
- Heme
- Reduced degradation
- Saccharomyces cerevisiae
- Bilirubin biosensor
- Heme
- Human hemoglobin
- Reduced degradation
- Saccharomyces cerevisiae
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Ishchuk, Olena, ...
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Frost, August T.
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Muniz, Facundo, ...
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Matsumoto, Saki, ...
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Laforge, Nathali ...
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Eriksson, Nélida ...
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visa fler...
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Martinez Ruiz, J ...
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Petranovic Niels ...
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Mikrobiologi
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Industriell biot ...
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och Annan industriel ...
- Artiklar i publikationen
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Metabolic Engine ...
- Av lärosätet
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Chalmers tekniska högskola
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Lunds universitet