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Sökning: id:"swepub:oai:research.chalmers.se:6c7a644e-82ed-47f6-b89e-2efadca83192" > Formation of Suppor...

Formation of Supported Lipid Bilayers at Surfaces with Controlled Curvatures: Influence of Lipid Charge

Sundh, M. (författare)
Århus Universitet,Aarhus University
Svedhem, Sofia, 1970 (författare)
Chalmers tekniska högskola,Chalmers University of Technology
Sutherland, D. S. (författare)
Århus Universitet,Aarhus University
 (creator_code:org_t)
2011-06-01
2011
Engelska.
Ingår i: Journal of Physical Chemistry B. - : American Chemical Society (ACS). - 1520-5207 .- 1520-6106. ; 115:24, s. 7838-7848
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • We have developed and characterized novel biomimetic membranes, formed at nanostructured sensor substrates with controlled curvatures, motivated by the many biological processes that involve membrane curvature. Model systems with convex nanostructures, with radii of curvatures (ROCs) of 70, 75, and 95 nm, were fabricated utilizing colloidal assembly and used as substrates for supported lipid bilayers (SLBs). The SLBs were formed via vesicle adsorption and rupture, and the vesicle deposition pathway was studied by means of quartz crystal microbalance with dissipation (QCM-D) and fluorescence microscopy. SLBs conforming to the underlying nanostructured surfaces, which exhibit increased surface area with decreased ROC, were confirmed from excess mass, monitored by QCM-D, and excess total fluorescence intensities. The formation of SLBs at the nanostructured surfaces was possible, however, depending on the ROC of the structures and the lipid vesicle charge the quality varied. The presence of nanostructures was shown to impair vesicle rupture and SLB formation was progressively hindered at surfaces with structures of decreasing ROCs. The introduction of a fraction of the positively charged lipid POEPC in the lipid vesicle membrane allowed for good quality and conformal bilayers at all surfaces. Alternatively, for vesicles formed from lipid mixtures with a fraction of the negatively charged lipid POPS, SLB formation was not at all possible at surfaces with the lowest ROC. Interestingly, the vesicle adsorption rate and the SLB formation were faster at surfaces with nanostructures of progressively smaller ROCs at high ratios of POPS in the vesicles. Development of templated SLBs with controlled curvatures provides a new experimental platform, especially at the nanoscale, at which membrane events such as lipid sorting, phase separation, and protein binding can be studied.

Ämnesord

NATURVETENSKAP  -- Kemi -- Fysikalisk kemi (hsv//swe)
NATURAL SCIENCES  -- Chemical Sciences -- Physical Chemistry (hsv//eng)

Nyckelord

phospholipid-bilayers
titanium-dioxide
phosphatidyl serine
phase
separation
quartz-crystal microbalance
qcm-d
vesicle adsorption
dissipation
membranes

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Av författaren/redakt...
Sundh, M.
Svedhem, Sofia, ...
Sutherland, D. S ...
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NATURVETENSKAP
NATURVETENSKAP
och Kemi
och Fysikalisk kemi
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Journal of Physi ...
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Chalmers tekniska högskola

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