Fungal Pretreatment of Oil Palm Empty Fruit Bunch: Effect of Manganese and Nitrogen

• Oil palm empty fruit bunch (OPEFB) was biologicallypretreated usingPleurotus floridanusLIPIMC996 supplementedwith various concentrations of manganese and nitrogen and incubated for 35 days at 30°C, and enzyme activities ofmanganese peroxidase (MnP) and laccase were examined. When OPEFB was supplemented with manganese, thehighest lignin reduction was achieved up to 25.0±5.6% at the addition of 200 μg Mn2+/g OPEFB.In addition,PleurotusfloridanusLIPIMC996 grew best on OPEFB supplemented with 800μg Mn2+/g OPEFB. When OPEFB wassupplemented with nitrogen, the highest lignin reduction was achieved up to 27.2± 3.5% at the additionof 20 mMnitrogen. The best growth ofPleurotus floridanuswas also achieved with the addition of 20 mM nitrogen. The additionof nitrogen and manganese on OPEFB did not significantly affect the activity of MnP and laccase.

Ämnesord

TEKNIK OCH TEKNOLOGIER  -- Industriell bioteknik -- Bioprocessteknik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Industrial Biotechnology -- Bioprocess Technology (hsv//eng)

Nyckelord

fungal growth

oil palm empty fruit bunch

lignino lytic enzymes

glucosamine

pretreatment

Pleurotus floridanus

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