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Protein engineering...
Protein engineering of invertase for enhancing yeast dough fermentation under high-sucrose conditions
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- Zhao, Yijin (författare)
- Beijing University of Chemical Technology,China Agricultural University
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- Meng, Kaiwen (författare)
- China Agricultural University
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- Fu, Jinyu (författare)
- Beijing University of Chemical Technology
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- Xu, Shijie (författare)
- Beijing University of Chemical Technology
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- Cai, Guang (författare)
- Beijing University of Chemical Technology
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- Meng, Geng (författare)
- China Agricultural University
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- Nielsen, Jens B, 1962 (författare)
- Beijing University of Chemical Technology,Chalmers tekniska högskola,Chalmers University of Technology
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- Liu, Zihe, 1984 (författare)
- Beijing University of Chemical Technology
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- Zhang, Yueping (författare)
- China Agricultural University
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(creator_code:org_t)
- 2022-10-06
- 2023
- Engelska.
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Ingår i: Folia Microbiologica. - : Springer Science and Business Media LLC. - 0015-5632 .- 1874-9356. ; 68:2, s. 207-217
- Relaterad länk:
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- During yeast dough fermentation, such as the high-sucrose bread-making process, the yeast cells are subjected to considerable osmotic stress, resulting in poor outcomes. Invertase is important for catalyzing the irreversible hydrolysis of sucrose to free glucose and fructose, and decreasing the catalytic activity of the invertase may reduce the glucose osmotic stress on the yeast. In this study, we performed structural design and site-directed mutagenesis (SDM) on the Saccharomyces cerevisiae invertase (ScInV) in an Escherichia coli expression system to study the catalytic activity of ScInV mutants in vitro. In addition, we generated the same mutation sites in the yeast endogenous genome and tested their invertase activity in yeast and dough fermentation ability. Our results indicated that appropriately reduced invertase activity of yeast ScInV can enhance dough fermentation activity under high-sucrose conditions by 52%. Our systems have greatly accelerated the engineering of yeast endogenous enzymes both in vitro and in yeast, and shed light on future metabolic engineering of yeast.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Annan industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Other Industrial Biotechnology (hsv//eng)
Nyckelord
- Site-directed mutagenesis
- Invertase activity
- Fermentation ability
- Protein engineering
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Zhao, Yijin
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Meng, Kaiwen
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Fu, Jinyu
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Xu, Shijie
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Cai, Guang
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Meng, Geng
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visa fler...
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Nielsen, Jens B, ...
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Liu, Zihe, 1984
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Zhang, Yueping
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Industriell biot ...
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och Biokatalys och e ...
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Industriell biot ...
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och Annan industriel ...
- Artiklar i publikationen
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Folia Microbiolo ...
- Av lärosätet
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Chalmers tekniska högskola