Sökning: id:"swepub:oai:research.chalmers.se:9463e2e8-8264-49e5-ada9-c592f0e20711" >
Production of 3-hyd...
Production of 3-hydroxypropionic acid from acetate using metabolically-engineered and glucose-grown Escherichia coli
-
- Lama, Suman (författare)
- Ulsan National Institute of Science and Technology (UNIST)
-
- Kim, Yeonhee (författare)
- Ulsan National Institute of Science and Technology (UNIST)
-
- Nguyen, Dat Tuan (författare)
- Ulsan National Institute of Science and Technology (UNIST)
-
visa fler...
-
- Im, Chaeho, 1989 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,Ulsan National Institute of Science and Technology (UNIST)
-
- Sankaranarayanan, Mugesh (författare)
- Vel Tech Institute of technology,Vel Tech Rangarajan Dr.Sagunthala R&D Institute of Science and Technology
-
- Park, Sunghoon (författare)
- Ulsan National Institute of Science and Technology (UNIST)
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2021
- 2021
- Engelska.
-
Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 320
- Relaterad länk:
-
https://research.cha...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Acetate can be used as carbon feedstock for the production of 3-hydroxypropionic acid (3-HP), but the production level was low due to inefficient cell growth on acetate. To better utilize acetate, a two-stage strategy, whereby glucose is used for cell growth and acetate for 3-HP formation, was attempted. Dissected malonyl-CoA reductase of Chloroflexus aurantiacus, alone or along with acetyl-CoA carboxylase and/or transhydrogenase, was overexpressed, and by-products formation pathway, glyoxylate shunt (GS) and gluconeogenesis were modified. When GS or gluconeogenesis was disrupted, cell growth on glucose was not hampered, while on acetate it was completely abolished. Consequently, 3-HP production, at production stage, were low, though 3-HP yield on acetate was increased, especially in the case of aceA deletion. In two-stage bioreactor, strain with upregulated GS produced 7.3 g/L 3-HP with yield of 0.26 mol/mol acetate. This study suggests that two-stage cultivation is a good strategy for 3-HP production from acetate.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Kemiteknik -- Kemiska processer (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Chemical Engineering -- Chemical Process Engineering (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Bioprocessteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bioprocess Technology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Annan industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Other Industrial Biotechnology (hsv//eng)
Nyckelord
- Engineered Escherichia coli
- 3-Hydroxypropionic acid
- Two-stage process
- Acetate utilization
- Malonyl-CoA reductase
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas