Studies of glycolytic flux regulation/control using permeabilised yeast cells

• In this work the correlation between levels of adenosine nucleotides and glycolytic flux was studied using a permeabilised cell system by experiments and in silico simulations using a kinetic model of glycolysis. Experimentally, using a flow-through chamber, the ATP level was correlated to glycolytic flux by a parabolic relation with a maximum at 2-3 mM of ATP. The kinetic model did not describe this parabolic correlation; at high ATP levels above 4 mM the flux was further increased. Thus, other mechanisms than described by the kinetic equations used in the model are important at high ATP levels to reduce the glycolytic flux.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

NATURVETENSKAP  -- Biologi -- Mikrobiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Microbiology (hsv//eng)

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