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Phospholipid membra...
Phospholipid membrane permeability of peptide nucleic acid
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- Wittung, Pernilla, 1968 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Kajanus, Johan, 1967 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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Edwards, K. (författare)
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Nielsen, P. (författare)
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- Nordén, Bengt, 1945 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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Malmstrom, B. G. (författare)
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(creator_code:org_t)
- 2000-03-07
- 1995
- Engelska.
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Ingår i: FEBS Letters. - : Wiley. - 1873-3468 .- 0014-5793. ; 365:1, s. 27-29
- Relaterad länk:
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http://dx.doi.org/10...
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- Phospholipid vesicles (liposomes) as membrane models have been used to study the penetration properties of peptide nucleic acid (PNA), a new DNA analog in which the nucleobases are attached to a pseudo-peptide backbone, The liposomes were characterised by carboxyfluorescein efflux, light-scattering and cryogenic transmission electron microscopy. The liposome structure was found not to be affected by the incorporation of PNA or an oligonucleotide. Two 10-mer fluorescein-labelled PNAs were found to have low efflux rates (half-times of 5.5 and 11 days), comparable to a 10-mer oligonucleotide (half-time of 7 days). We conclude that passive diffusion of unmodified PNA is not an effective way of transport into biological cells.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- oligonucleotide analogs
- pna
- thymine
- microscopy
- dna
- antisense
- vesicles
- liposome
- fluorescence
- peptide nucleic acid
- recognition
- cryogenic transmission electron
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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