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Dual-CARS microscopy
Dual-CARS microscopy
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- Enejder, Annika, 1969 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Brackmann, Christian, 1973 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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Burkacky, Ondrej (författare)
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visa fler...
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- Åkeson, Madeleine, 1981 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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visa färre...
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(creator_code:org_t)
- ISBN 9780819465559
- 2007
- 2007
- Engelska.
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Ingår i: PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE). - 0277-786X. - 9780819465559 ; 6442:15
- Relaterad länk:
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https://research.cha...
Abstract
Ämnesord
Stäng
- We present a new Coherent Anti-Stokes Raman Scattering (CARS) microscopy technique for label-free imaging of bio-molecules in living cells; dual-CARS microscopy. The use of three synchronized laser pulses in a dual-pump/dual-detection configuration enables imaging of two species with different molecular vibrations simultaneously, as well as acquisition of images free of non-resonant background. We show the power of the method by imaging deuterated nonadecane slowly diffusing into a suspension of living yeast cells in medium, clearly distinguishing the medium and the lipid droplets in the cells by probing the CH2 vibration from the D-nonadecane by probing the CD vibration. In addition, images of lipid stores in living C. elegans nematodes free of non-resonant background are shown. This results in a significant enhancement of the image contrast, allowing the visualization of emerging, low-density lipid stores in a dauer larva, difficult to distinguish in conventional CARS microscopy. The separation of the non-resonant background is shown to be beneficial also when monitoring molecules with weak vibrational modes. The improved sensitivity obtained is illustrated by probing the C=C vibration in polyunsaturated lipids extracted from fish. This enables the monitoring of the degree of unsaturation of lipids, a high value of which is reported in foods known to have positive effects on human health.
Ämnesord
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
Nyckelord
- Saccharomyces cerevisiae
- unsaturated lipids
- chemical imaging
- CARS
- vibrational imaging
- image contrast enhancement
- non-linear microscopy
- Caenorhabditis elegans
Publikations- och innehållstyp
- kon (ämneskategori)
- ref (ämneskategori)
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