Sökning: id:"swepub:oai:research.chalmers.se:ce9c42a4-8975-43a9-80e4-c0aaf9a3609e" >
Mirror-Image 5S Rib...
Mirror-Image 5S Ribonucleoprotein Complexes
-
- Ling, Jun-Jie (författare)
- Tsinghua University
-
- Fan, Chuyao (författare)
- Tsinghua University
-
- Qin, Hong (författare)
- Tsinghua University
-
visa fler...
-
- Wang, Min (författare)
- Tsinghua University
-
- Chen, Ji (författare)
- Tsinghua University
-
- Wittung Stafshede, Pernilla, 1968 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Zhu, Ting F. (författare)
- Tsinghua University
-
visa färre...
-
(creator_code:org_t)
- 2020-01-21
- 2020
- Engelska.
-
Ingår i: Angewandte Chemie - International Edition. - : Wiley. - 1433-7851 .- 1521-3773. ; 59:9, s. 3724-3731
- Relaterad länk:
-
https://research.cha... (primary) (free)
-
visa fler...
-
https://onlinelibrar...
-
https://research.cha...
-
https://doi.org/10.1...
-
https://research.cha...
-
visa färre...
Abstract
Ämnesord
Stäng
- After realizing mirror-image genetic replication, transcription, and reverse transcription, the biggest challenge in establishing a mirror-image version of the central dogma is to build a mirror-image ribosome-based translation machine. Here, we chemically synthesized the natural and mirror-image versions of three ribosomal proteins (L5, L18, and L25) in the large subunit of the Escherichia coli ribosome with post-translational modifications. We show that the synthetic mirror-image proteins can fold in vitro despite limited efficiency and assemble with enzymatically transcribed mirror-image 5S ribosomal RNA into ribonucleoprotein complexes. In addition, the RNA-protein interactions are chiral-specific in that the mirror-image ribosomal proteins do not bind with natural 5S ribosomal RNA and vice versa. The synthesis and assembly of mirror-image 5S ribonucleoprotein complexes are important steps towards building a functional mirror-image ribosome.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
- NATURVETENSKAP -- Data- och informationsvetenskap -- Datorseende och robotik (hsv//swe)
- NATURAL SCIENCES -- Computer and Information Sciences -- Computer Vision and Robotics (hsv//eng)
Nyckelord
- solid-phase peptide synthesis
- chemical protein synthesis
- chirality
- ribonucleoproteins
- native chemical ligation
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas