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Simultaneous maximi...
Simultaneous maximization of cell permeabilization and viability in single-cell electroporation using an electrolyte-filled capillary
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- Agarwal, A. (författare)
- University of Pittsburgh
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- Zudans, I. (författare)
- University of Pittsburgh
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- Orwar, Owe, 1964 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Weber, S. G. (författare)
- University of Pittsburgh
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(creator_code:org_t)
- 2006-12-02
- 2007
- Engelska.
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Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 79:1, s. 161-167
- Relaterad länk:
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https://europepmc.or...
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https://research.cha...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- A549 cells were briefly exposed to Thioglo-1, which converts thiols to fluorescent adducts. The fluorescent cells were exposed to short (50-300 ms) electric field pulses (500 V across a 15 cm capillary) created at the tip of an electrolyte-filled capillary. Fluorescence microscopy revealed varying degrees of cell permeabilization depending on the conditions. Longer pulses and a shorter cell-capillary tip distance led to a greater decrease in the cell's fluorescence. Live/dead (calcein AM and propidium iodide) testing revealed that a certain fraction of cells died. Longer pulses and shorter cell-capillary tip distances were more deadly. An optimum condition exists at a cell-capillary tip distance of 3.5-4.5 mu m and a pulse duration of 120-150 ms. At these conditions, > 90% of the cells are permeabilized and 80-90% survive.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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