Sökning: id:"swepub:oai:research.chalmers.se:d4a5a2b4-2a8a-4902-9b6f-f5311e0d6b2d" >
Influence of phase ...
Influence of phase separating lipids on supported lipid bilayer formation at SiO2 surfaces
-
- Sundh, M. (författare)
- Århus Universitet,Aarhus University
-
- Svedhem, Sofia, 1970 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Sutherland, D. S. (författare)
- Århus Universitet,Aarhus University
-
(creator_code:org_t)
- 2010
- 2010
- Engelska.
-
Ingår i: Physical Chemistry Chemical Physics. - : Royal Society of Chemistry (RSC). - 1463-9084 .- 1463-9076. ; 12:2, s. 453-460
- Relaterad länk:
-
http://dx.doi.org/10...
-
visa fler...
-
https://doi.org/10.1...
-
https://research.cha...
-
visa färre...
Abstract
Ämnesord
Stäng
- The importance of the lipid phase on the formation of supported lipid bilayers (SLBs) via vesicle fusion and on the resulting SLB homogeneity at SiO2 surfaces has been studied by the quartz crystal microbalance with dissipation (QCM-D) monitoring technique. Physiologically relevant lipid compositions were chosen to correspond to different regions (l(d), l(o) and coexistence of phases) in established phase diagrams of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), N-palmitoyl-D-erythro-sphingosylphosphorylcholine (PSM) and cholesterol. For most compositions, SLBs formed through vesicle rupture in a critical-surface-coverage dependent manner. Inclusion of PSM and cholesterol into POPC vesicles significantly impaired the vesicle rupture process such that a higher critical concentration of vesicles on the surface was needed before the rupture process started. When increasing the cholesterol content the vesicles formed SLBs containing more defects in the form of intact vesicles adsorbed on the surface up to a point (lo phase) where vesicles did not break at all but formed supported vesicular layers. The hampering of vesicle rupture is interpreted in terms of the ability of cholesterol to accommodate vesicle deformation. Experiments using elevated temperatures to alter the lipid membrane into a more fluid phase significantly improved the quality of the SLB showing the importance of both cholesterol content and the lipid phase on SLB homogeneity.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Annan teknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Other Engineering and Technologies (hsv//eng)
Nyckelord
- CELL-MEMBRANES
- BIOLOGICAL-MEMBRANES
- PHOSPHOLIPID-BILAYERS
- VESICLE ADSORPTION
- RAFTS
- PLASMON RESONANCE
- DOMAINS
- QUARTZ-CRYSTAL MICROBALANCE
- MODEL MEMBRANES
- MASS-SPECTROMETRY
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas