Sökning: id:"swepub:oai:research.chalmers.se:e6586fb0-129d-4d4b-89f5-fef9177c6e63" >
Reconstruction of t...
Reconstruction of the yeast Snf1 kinase regulatory network reveals its role as a global energy regulator
-
- Usaite, Renata (författare)
- Danmarks Tekniske Universitet,Technical University of Denmark,Scripps Research Institute
-
- Jewett, Michael C (författare)
- Danmarks Tekniske Universitet,Technical University of Denmark,Harvard Medical School
-
- Oliveira, Ana Paula (författare)
- Danmarks Tekniske Universitet,Technical University of Denmark,Eidgenössische Technische Hochschule Zürich (ETH),Swiss Federal Institute of Technology in Zürich (ETH)
-
visa fler...
-
- Yates, John R. (författare)
- Scripps Research Institute
-
- Olsson, Lisbeth, 1963 (författare)
- Danmarks Tekniske Universitet,Technical University of Denmark,Chalmers tekniska högskola,Chalmers University of Technology
-
- Nielsen, Jens B, 1962 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,Danmarks Tekniske Universitet,Technical University of Denmark
-
visa färre...
-
(creator_code:org_t)
- 2009-11-03
- 2009
- Engelska.
-
Ingår i: Molecular Systems Biology. - : EMBO. - 1744-4292. ; 5:319, s. 1-12
- Relaterad länk:
-
http://dx.doi.org/10...
-
visa fler...
-
https://doi.org/10.1...
-
https://research.cha...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Highly conserved among eukaryotic cells, ghe AMP-activated kinase (AMPK) is a central regulator of carbon metabolism. To map the complete network of interactions around AMPK in yeast (Snf1) and to evaluate the role of its regulatory subunit Snf4, we measured global mRNA, protein and metabolite levels in wild type, ∆snf1, ∆snf4, and ∆snf1∆snf4 knockout strains. Using four newly developed computional tools, including novel DOGMA sub-network analysis, we showed the benefits of three-level ome-data integration to uncover the global Snf1 kinase role in yeast. We for the first time identified Snf1's global regulation on gene and protein expression levels, and showed that yeast Snf1 has a far more extensive function in controlling energy metabolism that reported earlier. Additionally, we identified complementary roles of Snf1 and Snf4. Similar to the function of AMPK in humans, our findings showed that Snf1 is a low-energy checkpoint and that yeast can be used more extensively as a model system for studying the molecular mechanisms underlying the global regulation of AMPK in mammals, failure of which leads to metabolic diseases.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas