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Chiral Alcohols fro...
Chiral Alcohols from Alkenes and Water: Directed Evolution of a Styrene Hydratase
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- Gajdoš, Matúš (författare)
- Universität Bielefeld,Bielefeld University
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- Wagner, Jendrik (författare)
- Universität Bielefeld,Bielefeld University
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- Ospina, Felipe (författare)
- Universität Bielefeld,Bielefeld University
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- Köhler, Antonia (författare)
- Universität Bielefeld,Bielefeld University
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- Engqvist, Martin, 1983 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Hammer, Stephan C. (författare)
- Universität Bielefeld,Bielefeld University
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(creator_code:org_t)
- 2023-01-11
- 2023
- Engelska.
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Ingår i: Angewandte Chemie - International Edition. - : Wiley. - 1433-7851 .- 1521-3773. ; 62:7
- Relaterad länk:
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https://research.cha... (primary) (free)
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https://research.cha...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Enantioselective synthesis of chiral alcohols through asymmetric addition of water across an unactivated alkene is a highly sought-after transformation and a big challenge in catalysis. Herein we report the identification and directed evolution of a fatty acid hydratase from Marinitoga hydrogenitolerans for the highly enantioselective hydration of styrenes to yield chiral 1-arylethanols. While directed evolution for styrene hydration was performed in the presence of heptanoic acid to mimic fatty acid binding, the engineered enzyme displayed remarkable asymmetric styrene hydration activity in the absence of the small molecule activator. The evolved styrene hydratase provided access to chiral alcohols from simple alkenes and water with high enantioselectivity (>99 : 1 e.r.) and could be applied on a preparative scale.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
Nyckelord
- Hydratase
- Directed Evolution
- Biocatalysis
- Stereoselective Catalysis
- Alkene Hydration
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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