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Elucidating the con...
Elucidating the contributions of multiple aldehyde/alcohol dehydrogenases to butanol and ethanol production in Clostridium acetobutylicum
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- Dai, Zongijie, 1986 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Dong, H. J. (författare)
- Chinese Academy of Sciences
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- Zhang, Yueping (författare)
- Chinese Academy of Sciences
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- Li, Y. (författare)
- Chinese Academy of Sciences
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(creator_code:org_t)
- 2016-06-20
- 2016
- Engelska.
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322 .- 2045-2322. ; 6
- Relaterad länk:
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http://publications.... (primary) (free)
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https://www.nature.c...
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https://research.cha...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Ethanol and butanol biosynthesis in Clostridium acetobutylicum share common aldehyde/alcohol dehydrogenases. However, little is known about the relative contributions of these multiple dehydrogenases to ethanol and butanol production respectively. The contributions of six aldehyde/alcohol dehydrogenases of C. acetobutylicum on butanol and ethanol production were evaluated through inactivation of the corresponding genes respectively. For butanol production, the relative contributions from these enzymes were: AdhE1 > BdhB > BdhA approximate to YqhD > SMB_P058 > AdhE2. For ethanol production, the contributions were: AdhE1 > BdhB > YqhD > SMB_P058 > AdhE2 > BdhA. AdhE1 and BdhB are two essential enzymes for butanol and ethanol production. AdhE1 was relatively specific for butanol production over ethanol, while BdhB, YqhD, and SMB_P058 favor ethanol production over butanol. Butanol synthesis was increased in the adhE2 mutant, which had a higher butanol/ethanol ratio (8.15:1) compared with wild type strain (6.65:1). Both the SMB_P058 mutant and yqhD mutant produced less ethanol without loss of butanol formation, which led to higher butanol/ethanol ratio, 10.12:1 and 10.17:1, respectively. To engineer a more efficient butanol-producing strain, adhE1 could be overexpressed, furthermore, adhE2, SMB_P058, yqhD are promising gene inactivation targets. This work provides useful information guiding future strain improvement for butanol production.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- gene
- atcc 824
- purification
- escherichia-coli
- cultures
- Science & Technology - Other Topics
- fermentation
- electron flow
- genome sequence
- expression
- molecular characterization
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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