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Green Chemistry Pro...
Green Chemistry Production of Codlemone, the Sex Pheromone of the Codling Moth (Cydia pomonella), by Metabolic Engineering of the Oilseed Crop Camelina (Camelina sativa)
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- Xia, Yihan, 1990 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,Lunds universitet,Lund University
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- Wang, Hong Lei (författare)
- Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Feromongruppen,Forskargrupper vid Lunds universitet,Functional zoology,Department of Biology,Faculty of Science,Pheromone Group,Lund University Research Groups
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- Ding, Bao Jian (författare)
- Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Feromongruppen,Forskargrupper vid Lunds universitet,Functional zoology,Department of Biology,Faculty of Science,Pheromone Group,Lund University Research Groups
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- Svensson, Glenn P. (författare)
- Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Feromongruppen,Forskargrupper vid Lunds universitet,Functional zoology,Department of Biology,Faculty of Science,Pheromone Group,Lund University Research Groups
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- Jarl-Sunesson, Carin (författare)
- Lund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Molecular Cell Biology,Department of Biology,Faculty of Science
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- Cahoon, Edgar B. (författare)
- University of Nebraska - Lincoln
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- Hofvander, Per (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtförädling,Department of Plant Breeding,Sveriges lantbruksuniversitet (SLU),Swedish University of Agricultural Sciences (SLU)
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- Löfstedt, Christer (författare)
- Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Feromongruppen,Forskargrupper vid Lunds universitet,Functional zoology,Department of Biology,Faculty of Science,Pheromone Group,Lund University Research Groups
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- Xia, Yi Han (författare)
- Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Functional zoology,Department of Biology,Faculty of Science
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(creator_code:org_t)
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- 2021-11-11
- 2021
- Engelska.
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Ingår i: Journal of Chemical Ecology. - : Springer Science and Business Media LLC. - 1573-1561 .- 0098-0331. ; 47:12, s. 950-967
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Abstract
Ämnesord
Stäng
- Synthetic pheromones have been used for pest control over several decades. The conventional synthesis of di-unsaturated pheromone compounds is usually complex and costly. Camelina (Camelina sativa) has emerged as an ideal, non-food biotech oilseed platform for production of oils with modified fatty acid compositions. We used Camelina as a plant factory to produce mono- and di-unsaturated C12 chain length moth sex pheromone precursors, (E)-9-dodecenoic acid and (E,E)-8,10-dodecadienoic acid, by introducing a fatty acyl-ACP thioesterase FatB gene UcTE from California bay laurel (Umbellularia californica) and a bifunctional ∆9 desaturase gene Cpo_CPRQ from the codling moth, Cydia pomonella. Different transgene combinations were investigated for increasing pheromone precursor yield. The most productive Camelina line was engineered with a vector that contained one copy of UcTE and the viral suppressor protein encoding P19 transgenes and three copies of Cpo_CPRQ transgene. The T2 generation of this line produced 9.4% of (E)-9-dodecenoic acid and 5.5% of (E,E)-8,10-dodecadienoic acid of the total fatty acids, and seeds were selected to advance top-performing lines to homozygosity. In the T4 generation, production levels of (E)-9-dodecenoic acid and (E,E)-8,10-dodecadienoic acid remained stable. The diene acid together with other seed fatty acids were converted into corresponding alcohols, and the bioactivity of the plant-derived codlemone was confirmed by GC-EAD and a flight tunnel assay. Trapping in orchards and home gardens confirmed significant and specific attraction of C. pomonella males to the plant-derived codlemone.
Ämnesord
- LANTBRUKSVETENSKAPER -- Bioteknologi med applikationer på växter och djur -- Växtbioteknologi (hsv//swe)
- AGRICULTURAL SCIENCES -- Agricultural Biotechnology -- Plant Biotechnology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Zoologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Zoology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Jordbruksvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Agricultural Science (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Bioassay
- Agrobacterium-based floral-dip transformation
- Plant factory
- Acyl-ACP thioesterase
- Multi-gene copies
- P19
- ∆9 desaturase
- Conjugated double bonds
- Acyl-ACP thioesterase
- Agrobacterium-based floral-dip transformation
- Bioassay
- Conjugated double bonds
- Multi-gene copies
- P19
- Plant factory
- ∆9 desaturase
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Xia, Yihan, 1990
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Wang, Hong Lei
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Ding, Bao Jian
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Svensson, Glenn ...
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Jarl-Sunesson, C ...
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Cahoon, Edgar B.
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visa fler...
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Hofvander, Per
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Löfstedt, Christ ...
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Xia, Yi Han
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- Om ämnet
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- LANTBRUKSVETENSKAPER
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LANTBRUKSVETENSK ...
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och Bioteknologi med ...
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och Växtbioteknologi
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Zoologi
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Organisk kemi
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- LANTBRUKSVETENSKAPER
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LANTBRUKSVETENSK ...
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och Lantbruksvetensk ...
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och Jordbruksvetensk ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biokemi och mole ...
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Journal of Chemi ...
- Av lärosätet
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Chalmers tekniska högskola
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Sveriges Lantbruksuniversitet
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Lunds universitet