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Genome editing in p...
Genome editing in penicillium chrysogenum using cas9 ribonucleoprotein particles
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- Pohl, Carsten (författare)
- Rijksuniversiteit Groningen,University of Groningen
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- Mózsik, László (författare)
- Rijksuniversiteit Groningen,University of Groningen
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- Driessen, Arnold J.M. (författare)
- Rijksuniversiteit Groningen,University of Groningen
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- Bovenberg, R. A. L. (författare)
- Rijksuniversiteit Groningen,University of Groningen,DSM Biotechnology Centre
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- Nygård, Yvonne, 1986 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,Rijksuniversiteit Groningen,University of Groningen
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(creator_code:org_t)
- 2018-05-13
- 2018
- Engelska.
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Ingår i: Methods in Molecular Biology. - New York, NY : Springer New York. - 1940-6029 .- 1064-3745. ; , s. 213-232
- Relaterad länk:
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https://pure.rug.nl/...
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https://research.cha...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Several CRISPR/Cas9 tools have been recently established for precise genome editing in a wide range of filamentous fungi. This genome editing platform offers high flexibility in target selection and the possibility of introducing genetic deletions without the introduction of transgenic sequences. This chapter describes an approach for the transformation of Penicillium chrysogenum protoplasts with preassembled ribonucleoprotein particles (RNPs) consisting of purified Cas9 protein and in vitro transcribed single guide RNA (sgRNA) for the deletion of genome sequences or their replacement with alternative sequences. This method is potentially transferable to all fungal strains where protoplasts can be obtained from.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
Nyckelord
- Ribonucleoprotein particle
- HDR
- Penicillium chrysogenum
- Genome editing
- NHEJ
- CRISPR/Cas9
- RNP
- Filamentous fungi
Publikations- och innehållstyp
- kap (ämneskategori)
- vet (ämneskategori)
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