Highly efficient in vitro regeneration of the industrial oilseed crop Crambe abyssinica

• A highly efficient regeneration protocol for oilseed crop Crambe abyssinica has been developed using hypocotyls as explants in this study. Crambe is a potential engineering oilseed crop for industrial purposes as it contains 55-60% erucic acid in its oil and, more importantly, it does not outcross with any food oil seed crops. However, the low regeneration frequency with the currently available protocols is still a limiting factor for genetic modification of Crambe. In this study, we investigated the effects of N-source, C-source, AgNO(3), cultural conditions as well as the concentration and combination of plant growth regulators (PGR) on the regeneration frequency of C. abyssinica. The results showed that all these factors, especially the N-source and PGR concentrations and combinations, played an important role in shoot regeneration. Among all the factors tested, the combination of using hypocotyls from C. abyssinica cv. galactica, the Lepiovre basal medium supplemented with 16 g l(-1) glucose, 0.5 g l(-1) AgNO(3), 2.2 mg l(-1) thidiazuron (TDZ), 0.5 mg l(-1) alpha-naphthaleneacetic acid (NAA), 2.5 g l(-1) Gelrite, seeds germinated in dark for 3 days and explants cultured in light, gave the best regeneration frequency (over 95%). The results also suggest that reducing the content of NH(4)(+) or keeping a suitable NO(3)(-)/NH(4)(+). ratio in the regeneration medium would be crucial to Crambe shoot regeneration. (C) 2010 Elsevier B.V. All rights reserved.

Ämnesord

LANTBRUKSVETENSKAPER  -- Lantbruksvetenskap, skogsbruk och fiske -- Jordbruksvetenskap (hsv//swe)

AGRICULTURAL SCIENCES  -- Agriculture, Forestry and Fisheries -- Agricultural Science (hsv//eng)

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